Sonodynamically-induced anticancer effects by functionalized fullerenes.
Sonodynamically-induced anticancer effects by functionalized fullerenes.
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发表时间：
2013-08
影响因子：
2
通讯作者：
N. Yumita;Y. Iwase;Takahiro Imaizumi;Ai Sakurazawa;Yuuka Kaya;K. Nishi;T. Ikeda;S. Umemura;Fu-shih Chen;Y. Momose
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作者：
N. Yumita;Y. Iwase;Takahiro Imaizumi;Ai Sakurazawa;Yuuka Kaya;K. Nishi;T. Ikeda;S. Umemura;Fu-shih Chen;Y. Momose
背景功能化富勒烯，如多羟基富勒烯(PHF)，由于其水溶性和作为碳纳米材料在肿瘤成像和治疗中的潜在应用而引起了人们的特别关注。本研究探讨了PHF在声动力学诱导下的抗肿瘤作用。材料与方法采用体外培养的S180肉瘤细胞和结肠癌26细胞的实体瘤，研究PHF联合超声的声动力学抗肿瘤作用。结果在80μM PHF存在下，超声对细胞的损伤可增加一倍。组氨酸显著抑制这种增强。这种抑制作用表明，声动力诱导的抗肿瘤作用是由声动力产生的活性氧介导的。超声照射与PHF联合治疗可抑制26例结肠癌移植瘤的生长。超声联合PHF治疗可见肿瘤组织的破坏，而PHF单独治疗或单独超声治疗均未引起肿瘤组织坏死。结论PHF是一种潜在的声动力学治疗实体瘤的声敏剂。
BACKGROUND Functionalized fullerenes, such as polyhydroxy fullerenes (PHF), have attracted particular attention due to their water solubility and their potential application in tumor imaging and therapy as carbon nanomaterials. In this study, the sonodynamically-induced antitumor effect of PHF was investigated. MATERIALS AND METHODS Sonodynamically-induced antitumor effects of PHF in combination with ultrasound were investigated using isolated sarcoma 180 cells and solid tumor from colon 26 carcinoma cells. RESULTS The cell damage induced by sonication was enhanced by two-fold in the presence of 80 μM PHF. Histidine significantly inhibited this enhancement. This inhibitory effect suggests that the sonodynamically-induced antitumor effect was mediated by sonodynamically-generated reactive oxygen species. The combined treatment of ultrasonic exposure with PHF suppressed the growth of implanted colon 26 tumors. The destruction of tumor tissue was observed with the ultrasonic treatment in combination with PHF, while neither the treatment with PHF alone nor that with ultrasound alone caused necrosis. CONCLUSION These results suggest that PHF is a potential sonosensitizer for sonodynamic treatment of solid tumors.