Development and characterization of polymorphic microsatellite markers in Cyclobalanopsis glauca (Fagaceae).
Development and characterization of polymorphic microsatellite markers in Cyclobalanopsis glauca (Fagaceae).
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DOI：
10.3732/ajb.1100448
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发表时间：
2012-03
影响因子：
3
通讯作者：
Xin Tong;N. Xu;Ling Li;Xiao‐Yong Chen
中科院分区：
文献类型：
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作者：
Xin Tong;N. Xu;Ling Li;Xiao‐Yong Chen
本研究以万年青阔叶雌雄同株乔木青冈为研究对象，分离并鉴定了青冈的多态性微卫星位点，为分析青冈的亲缘关系和交配系统提供了工具。方法与结果开发了13个多态性微卫星标记，并在3个C. glauca种群。每个位点的等位基因数从2个到22个不等。群体内的观测杂合度和期望杂合度分别为0.000-0.967和0.033-0.949。结论所设计的多态性引物在供试群体内表现出较高的多态性水平，可用于中国仓鼠的亲缘关系分析和交配系统估计。glauca。
PREMISE OF THE STUDY We isolated and characterized polymorphic microsatellite loci in Cyclobalanopsis glauca (Fagaceae), an evergreen broadleaved monoecious tree, to provide tools for analyzing parentage and mating system. METHODS AND RESULTS Thirteen polymorphic microsatellite markers were developed and tested in three C. glauca populations. The number of alleles per locus varied from two to 22. The observed and expected heterozygosities within populations were 0.000-0.967 and 0.033-0.949, respectively. CONCLUSIONS These polymorphic primers showed high levels of polymorphism within tested populations, and can be used in parentage analysis and mating system estimation of C. glauca.