In Vivo Vascular Network Forming Assay.
In Vivo Vascular Network Forming Assay.
复制标题
DOI：
10.1007/978-1-0716-0916-3_14
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Melero-Martin JM
中科院分区：
文献类型：
--
作者：
Kim HD;Lin RZ;Melero-Martin JM
形成功能性血管网络的能力对于内皮细胞的表征至关重要。在这一章中，我们将审查一个修改后的体内血管网络形成试验，取代传统的小鼠肿瘤来源的基质胶与定义明确的胶原蛋白纤维蛋白水凝胶。该测定是可靠的，并且不需要特殊设备、外科手术或技术人员来执行。此外，研究人员可以根据需要修改这种方法，用于测试不同的细胞来源，基因功能，生长因子和药物分子的干扰，以及用于开发和研究增强工程化人体组织和器官的新血管形成的策略。
The capability of forming functional blood vessel networks is critical for the characterization of endothelial cells. In this chapter, we will review a modified in vivo vascular network forming assay by replacing traditional mouse tumor-derived Matrigel with a well-defined collagen–fibrin hydrogel. The assay is reliable and does not require special equipment, surgical procedure, or a skilled person to perform. Moreover, investigators can modify this method on-demand for testing different cell sources, perturbation of gene functions, growth factors, and pharmaceutical molecules, and for the development and investigation of strategies to enhance neovascularization of engineered human tissues and organs.