Fungicidal components of mammalian granulocytes active against Cryptococcus neoformans.
Fungicidal components of mammalian granulocytes active against Cryptococcus neoformans.
复制标题
哺乳动物粒细胞的杀菌成分对新型隐球菌具有活性。
DOI：
10.1093/infdis/136.1.96
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
K. M. Ladra
中科院分区：
文献类型：
--
作者：
R. Lehrer;K. M. Ladra
从兔、豚鼠嗜中性粒细胞或人中性粒细胞制备的富含颗粒级分的柠檬酸提取物在体外对新生隐球菌具有杀灭作用。这些提取物用聚丙烯酰胺凝胶微制备电泳法进行分级。在制备兔和豚鼠异嗜性细胞时，隐球菌的杀菌活性主要与溶酶体阳离子蛋白复合体有关。人类中性粒细胞缺乏严格可比的阳离子蛋白，但含有杀死新生葡萄球菌的其他成分。这些成分似乎与先前描述的人类中性粒细胞对近平假丝酵母菌有效的蛋白质相同。
Citric acid extracts of granule-rich fractions, prepared from rabbit and guinea pig heterophils or human neutrophis, killed Cryptococcus neoformans in vitro. These extracts sere fractionated by micropreparative electrophoresis on polyacrylamide gels. In preparation of rabbit and guinea pig heterophils, cryptococcidal activity was associated predominantely with lysosomal cationic protein complex. Human neutrophils lacked strictly comparable cationic proteins but contained other components that killed C. neoformans. These components appeared to be identical to previously described proteins of the human neutrophil active against Candida parapsilosis.