Interspecies and intraspecies transmission of triple reassortant H3N2 influenza A viruses.
Interspecies and intraspecies transmission of triple reassortant H3N2 influenza A viruses.
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DOI：
10.1186/1743-422x-4-129
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发表时间：
2007-11-28
期刊：
影响因子：
4.8
通讯作者：
Saif YM
中科院分区：
文献类型：
--
作者：
Yassine HM;Al-Natour MQ;Lee CW;Saif YM
三重重组 H3N2 病毒于 1998 年首次从猪身上分离出来，已知在美国猪和火鸡种群中流行。 2004 年，我们从俄亥俄州和伊利诺伊州的两个火鸡种鸡群中分离出两种 H3N2 三重重组病毒。受感染的母鸡没有表现出临床症状，但产蛋完全停止。在这项研究中，我们评估了三种源自火鸡的三重重配 H3N2 分离株和一种源自猪的分离株在猪和火鸡之间的传播情况。尽管测试的所有 4 种病毒在所有 8 个基因中都具有高度遗传相似性，但只有俄亥俄毒株 (A/turkey/Ohio/313053/04) 被证明可以在猪和火鸡之间有效地双向传播。一种分离株 A/turkey/North Carolina/03 能够从猪传播给火鸡，但反之则不然。其他两种病毒都没有以任何一种方式传播。对俄亥俄菌株 HA1 基因的序列分析显示，从火鸡传播到猪后，受体结合结构域的残基 190 处出现一个氨基酸变化（D 变为 A）。然后对俄亥俄州病毒在三种不同鸟类中的种内传播进行了测试。该病毒被证明可以在火鸡中复制和传播，在鸡中复制但不传播，并且不在鸭中复制。识别具有不同种间和种内传播潜力的病毒应该有助于进一步研究种间传播的分子基础。
The triple reassortant H3N2 viruses were isolated for the first time from pigs in 1998 and are known to be endemic in swine and turkey populations in the United States. In 2004, we isolated two H3N2 triple reassortant viruses from two turkey breeder flocks in Ohio and Illinois. Infected hens showed no clinical signs, but experienced a complete cessation of egg production. In this study, we evaluated three triple reassortant H3N2 isolates of turkey origin and one isolate of swine origin for their transmission between swine and turkeys. Although all 4 viruses tested share high genetic similarity in all 8 genes, only the Ohio strain (A/turkey/Ohio/313053/04) was shown to transmit efficiently both ways between swine and turkeys. One isolate, A/turkey/North Carolina/03, was able to transmit from pigs to turkeys but not vice versa. Neither of the other two viruses transmitted either way. Sequence analysis of the HA1 gene of the Ohio strain showed one amino acid change (D to A) at residue 190 of the receptor binding domain upon transmission from turkeys to pigs. The Ohio virus was then tested for intraspecies transmission in three different avian species. The virus was shown to replicate and transmit among turkeys, replicate but does not transmit among chickens, and did not replicate in ducks. Identifying viruses with varying inter- and intra-species transmission potential should be useful for further studies on the molecular basis of interspecies transmission.