Proteomic Analysis Reveals Key Proteins and Phosphoproteins upon Seed Germination of Wheat (Triticum aestivum L.).
Proteomic Analysis Reveals Key Proteins and Phosphoproteins upon Seed Germination of Wheat (Triticum aestivum L.).
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DOI：
10.3389/fpls.2015.01017
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发表时间：
2015
影响因子：
5.6
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Dong K;Zhen S;Cheng Z;Cao H;Ge P;Yan Y
小麦（Triticum aestivum L.）是世界上最古老的种植作物之一，也是世界上第二重要的粮食作物。种子萌发是植物生长发育的关键发育过程，萌发不良直接影响植物的生长和随后的籽粒产量。在这项研究中，我们首次使用基于二维差分凝胶电泳（2D-DIGE）的蛋白质组学方法对小麦种子萌发进行了动态蛋白质组学分析。共鉴定出166个差异表达蛋白（differential expression protein， DEP）位点，代表73个独特的蛋白，这些蛋白主要参与贮藏、胁迫/防御/解毒、碳水化合物代谢、光合作用、细胞代谢和转录/翻译/转位。所鉴定的DEPs及其动态表达谱通常对应于吸吸后种子萌发的三个不同阶段：储存降解、生理过程/形态发生和光合作用。一些参与贮藏物质降解和植物防御机制的关键DEPs，如球蛋白3、蔗糖合酶I型、丝氨酸蛋白酶、β -淀粉酶和质体adp -葡萄糖焦磷酸化酶（AGPase）小亚基，在种子萌发过程中被磷酸化。特别是，发现磷酸化位点Ser355位于β -淀粉酶的酶活性区域，促进底物结合。几种蛋白的磷酸化修饰可促进种子萌发过程中贮藏物质的降解和环境胁迫防御。本文提出了小麦种子萌发的核心代谢途径，为研究谷物种子萌发的分子机制提供了新的思路。
Wheat (Triticum aestivum L.) is one of the oldest cultivated crops and the second most important food crop in the world. Seed germination is the key developmental process in plant growth and development, and poor germination directly affects plant growth and subsequent grain yield. In this study, we performed the first dynamic proteome analysis of wheat seed germination using a two-dimensional differential gel electrophoresis (2D-DIGE)-based proteomic approach. A total of 166 differentially expressed protein (DEP) spots representing 73 unique proteins were identified, which are mainly involved in storage, stress/defense/detoxification, carbohydrate metabolism, photosynthesis, cell metabolism, and transcription/translation/transposition. The identified DEPs and their dynamic expression profiles generally correspond to three distinct seed germination phases after imbibition: storage degradation, physiological processes/morphogenesis, and photosynthesis. Some key DEPs involved in storage substance degradation and plant defense mechanisms, such as globulin 3, sucrose synthase type I, serpin, beta-amylase, and plastid ADP-glucose pyrophosphorylase (AGPase) small subunit, were found to be phosphorylated during seed germination. Particularly, the phosphorylation site Ser355 was found to be located in the enzyme active region of beta-amylase, which promotes substrate binding. Phosphorylated modification of several proteins could promote storage substance degradation and environmental stress defense during seed germination. The central metabolic pathways involved in wheat seed germination are proposed herein, providing new insights into the molecular mechanisms of cereal seed germination.