MAST CELLS IN RAT GASTROINTESTINAL MUCOSA .I. EFFECTS OF FIXATION

MAST CELLS IN RAT GASTROINTESTINAL MUCOSA .I. EFFECTS OF FIXATION
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DOI:
10.1111/apm.1966.66.3.289
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发表时间:
1966-01-01
期刊:
ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA
影响因子:
--
通讯作者:
ENERBACK, L
ENERBACK, L
中科院分区:
其他
文献类型:
--
作者:
ENERBACK, L

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细胞经4%甲醛固定后不能显示,经0。4-0. 8%甲醛显然,肠肥大细胞的颗粒在组织学过程中比其他肥大细胞的颗粒更容易溶解。一种含有0. 6%甲醛和0. 5%乙酸对两种肥大细胞的甲苯胺蓝染色均显示良好的保存效果。
The cells could not be demonstrated after fixation in 4% formaldehyde, but their stainability with toluidine blue was preserved after fixation in 0. 4-0. 8% formaldehyde. Apparently the granules of intestinal mast cells are more readily dissolved during histological processes than those of other mast cells. A fixative containing 0. 6% formaldehyde and 0. 5% acetic acid gave good preservation of stainability with toluidine blue of both types of mast cells in the rat.