NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARD.
NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARD.
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DOI：
10.1083/jcb.25.2.327
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发表时间：
1965-05
期刊：
影响因子：
--
通讯作者：
BOGORAD L
中科院分区：
文献类型：
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作者：
KISLEV N;SWIFT H;BOGORAD L
用光学显微镜和电子显微镜技术研究了瑞士甜菜幼叶中的核酸。叶DNA的特征还在于密度梯度离心，并显示含有较高的鸟嘌呤加胞嘧啶（GC）含量的次要带，推测可归因于叶绿体。叶绿体被微弱的Feulgen反应染色;放射自显影显示氚化胸苷在细胞质和一些细胞核中的掺入。Feulgen β和大部分放射性可用DNA酶去除。在电子显微镜下，线粒体和叶绿体被发现含有丝状和颗粒成分内的矩阵区域。丝状组分的形态取决于固定，在OSO 4或福尔马林后部分聚集，但在Kellenberger固定后细丝状。用乙酸双氧铀处理细丝，并在提取核DNA的条件下用DNA酶在福尔马林固定后提取。颗粒成分，福尔马林固定和铀酰染色后，是突出的叶绿体从年轻的叶片，但只有稀疏分布在线粒体。用核糖核酸酶除去酶。我们得出的结论是，瑞士甜菜的叶绿体和线粒体具有丝状组分，含有DNA，可能负责细胞质胸苷掺入和氯化铯离心的次要带。还存在含有RNA的颗粒状核糖体样组分。
Nucleic acids in young leaves of Swiss chard have been studied by light and electron microscope techniques. Leaf DNA has also been characterized by density gradient centrifugation and shown to contain a minor band of higher guanine plus cytosine (GC) content, presumably attributable to chloroplasts. The chloroplasts were faintly stained by the Feulgen reaction; radioautography demonstrated the incorporation of tritiated thymidine in the cytoplasm and in some nuclei. The Feulgen stainability and most of the radioactivity were removable with DNase. Under the electron microscope, both mitochondria and chloroplasts were found to contain filamentous and particulate components within the matrix areas. The morphology of the filamentous component was dependent on the fixation, being partially clumped after OSO4 or formalin, but finely filamentous after Kellenberger fixation. The filaments were stainable with uranyl acetate, and were extractable with DNase following formalin fixation under conditions in which nuclear DNA was also extracted. The particulate component, after formalin fixation and uranyl staining, was prominent in chloroplasts from young leaves, but was only sparsely distributed in mitochondria. The stainability was removed with ribonuclease. We have concluded that chloroplasts and mitochondria of Swiss chard possess a filamentous component that contains DNA, probably responsible for both cytoplasmic thymidine incorporation and the minor band in CsCl centrifugation. A particulate ribosome-like component that contains RNA is also present.