The comparative effects of mesoporous silica nanoparticles and colloidal silica on inflammation and apoptosis
The comparative effects of mesoporous silica nanoparticles and colloidal silica on inflammation and apoptosis
复制标题
DOI：
10.1016/j.biomaterials.2011.08.042
复制
发表时间：
2011-12-01
期刊：
影响因子：
14
通讯作者：
Kim, Sang-Hyun
中科院分区：
文献类型：
--
作者：
Lee, Soyoung;Yun, Hui-Suk;Kim, Sang-Hyun
介孔氧化硅（MPS）是一种新型的纳米材料，在药物释放、标记、组织工程等领域具有广泛的应用前景。MPS与普通二氧化硅（胶体二氧化硅）之间的显著差异在于孔结构，如比表面积和孔体积。纳米材料的孔结构被认为是导致纳米毒性的关键条件之一，因为它们的细胞摄取和免疫应答效率不同。我们首先研究了二氧化硅纳米颗粒的孔结构条件对炎症和细胞凋亡的影响，在体外和体内，通过比较MPS和胶体二氧化硅，并定义了潜在的作用机制。MPS和胶态二氧化硅纳米颗粒都是通过几乎相似的合成条件产生的，除了使用用于MPS的聚合物模板。胶体二氧化硅和MPS的比表面积分别为40和1150 m2/g，而其他条件，包括粒径（100 nm）和形状（球形），保持不变。在MTT测定和FACS分析中，MPS纳米颗粒显示出比胶体二氧化硅纳米颗粒显著更少的细胞毒性和凋亡细胞死亡。MPS纳米颗粒诱导巨噬细胞中促炎细胞因子如肿瘤坏死因子-α、白细胞介素（IL）-β和IL-6的表达降低。MPS纳米颗粒引起的炎症反应和细胞凋亡减少是由于丝裂原活化蛋白激酶、核因子-κ B和半胱天冬酶3减少所致。此外，使用局部淋巴结试验（一种用于接触性超敏反应危害识别的独立体内方法），我们表明胶体二氧化硅纳米颗粒可作为免疫原性致敏剂并诱导接触性超敏反应，但MPS纳米颗粒则不然。总之，二氧化硅纳米颗粒的孔结构极大地影响其生物相容性，应仔细设计。MPS纳米颗粒表现出比胶体二氧化硅更好的生物相容性，并有望在生物医学和生物技术应用领域中获得优异的潜在用途。(C)2011爱思唯尔有限公司版权所有。
Mesoporous silica (MPS), synthesized via the supramolecular polymer templating method, is one of the most attractive nanomaterials for biomedical applications, such as drug delivery systems, labeling, and tissue engineering. The significant difference between MPS and general silica (colloidal silica) is the pore architectures, such as specific surface area and pore volume. The pore structures of nanomaterials have been considered to be one of the key conditions, causing nanotoxicity due to their different efficiency of cellular uptake and immune response. We first studied the influence of pore structural conditions of silica nanoparticles on both inflammation and apoptosis, in vitro and in vivo, by comparing MPS and colloidal silica, and defined underlying mechanisms of action. Both the MPS and colloidal silica nanoparticles are produced by almost similar synthetic conditions, except the use of polymer template for MPS. The specific surface area of colloidal silica and MPS was 40 and 1150 m(2) g(-1), respectively, while other conditions, including particle size (100 nm) and shape (spherical), were kept constant. In both MTT assay and FACS analysis, MPS nanoparticles showed significantly less cytotoxicity and apoptotic cell death than colloidal silica nanoparticles. MPS nanoparticles induced lower expression of pro-inflammatory cytokines, such as tumor necrosis factor-alpha, interleukin (IL)-beta, and IL-6, in macrophages. The reduced inflammatory response and apoptosis elicited by MPS nanoparticles were resulting from the reduction of mitogen-activated protein kinases, nuclear factor-kappa B, and caspase 3. In addition, using the local lymph node assay, a standalone in vivo method for hazard identification of contact hypersensitivity, we showed that colloidal silica nanoparticles act as an immunogenic sensitizer and induce contact hypersensitivity but not MPS nanoparticles. In conclusion, the pore architecture of silica nanoparticles greatly influences their biocompatibility and should be carefully designed. The MPS nanoparticles exhibit better biocompatibility than colloidal silica and promise excellent potential usage in the field of biomedical and biotechnological applications. (C) 2011 Elsevier Ltd. All rights reserved.