Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation.
Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation.
复制标题
人类诱导多能干细胞 (iPSC) 处理方案：维护、扩增和冷冻保存。
DOI：
10.1007/7651_2021_358
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Fossati,Valentina
中科院分区：
文献类型：
--
作者：
Marotta,Davide;Rao,Chandrika;Fossati,Valentina
近年来，人类诱导多能干细胞（iPSC）已成为基础研究，疾病建模和药物发现的宝贵资源。鉴于iPSC相对于替代模型的众多优势-包括其人类起源，其分化成几乎任何细胞类型的能力以及患者特异性iPSC在个性化医疗中的治疗潜力-许多实验室现在正在考虑iPSC模型用于他们的研究。由于iPSC起始群体的质量是任何一种应用成功的关键决定因素，因此坚持iPSC培养的最佳实践至关重要。在以下方案中，我们提供了建立和维持高质量iPSC培养物所需的培养、冷冻保存和质量控制方法的全面指南。
Human induced pluripotent stem cells (iPSCs) have emerged as an invaluable resource for basic research, disease modeling, and drug discovery over recent years. Given the numerous advantages of iPSCs over alternative models—including their human origin, their ability to be differentiated into almost any cell type, and the therapeutic potential of patient-specific iPSCs in personalized medicine—many labs are now considering iPSC models for their studies. As the quality of the starting population of iPSCs is a key determinant in the success of any one of these applications, it is crucial to adhere to best practices in iPSC culture. In the following protocol, we offer a comprehensive guide to the culture, cryopreservation, and quality control methods required for the establishment and maintenance of high-quality iPSC cultures.