Evaluation of pain-related behavior, bone destruction and effectiveness of fentanyl, sufentanil, and morphine in a murine model of cancer pain
Evaluation of pain-related behavior, bone destruction and effectiveness of fentanyl, sufentanil, and morphine in a murine model of cancer pain
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DOI：
10.1016/j.pbb.2005.07.016
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发表时间：
2005-09-01
影响因子：
3.6
通讯作者：
Meert, TF
中科院分区：
文献类型：
--
作者：
El Mouedden, M;Meert, TF
本研究旨在评估骨癌疼痛小鼠模型中骨癌生长过程中的疼痛发展和骨破坏情况，并评估芬太尼、舒芬太尼和吗啡在该模型中的镇痛效果。将C3H/HeNCrl小鼠的股骨骨髓腔内接种溶骨性NCTC 2472纤维肉瘤细胞，并在3周内观察以评估骨癌生长过程中的疼痛行为（在转棒上强制行走时自发抬腿和肢体使用情况）和骨破坏（通过对荷瘤骨进行微CT扫描确定的骨损伤相关参数）。结果表明，在这种癌症诱导的骨痛小鼠模型中，疼痛的行为表现与骨破坏的进展同时出现。在接种后第15天和第22天皮下给予芬太尼（0.025 - 0.64 mg/kg）、舒芬太尼（0.005 - 0.04 mg/kg）和吗啡（2.5 - 40 mg/kg），可剂量依赖性地减轻疼痛相关行为。以下剂量可完全缓解疼痛相关行为：≥0.16 mg/kg芬太尼、0.02 mg/kg舒芬太尼和20 mg/kg吗啡。总之，结果显示肿瘤生长诱导的骨破坏与行为性疼痛表现之间存在明确联系，后者可被阿片类药物芬太尼、舒芬太尼和吗啡有效控制。（c）2005爱思唯尔公司。保留所有权利。
The present study was conducted to evaluate the pain development and bone destruction during bone cancer growth in a murine model of bone cancer pain and to evaluate the analgesic efficacy of fentanyl, sufentanil, and morphine in this model. C3H/HeNCrl mice were inoculated into the intramedullary space of the femur with osteolytic NCTC 2472 fibrosarcoma cells, and followed during a 3-week period to assess pain behaviors (spontaneous lifting and limb-use during forced ambulation on rotarod) and bone destruction (parameters indicative of bone lesions determined by mu CT-scans of the tumor-bearing bones) during bone cancer growth. The results showed that in this murine model of cancer-induced bone pain, behavioural manifestations of pain emerge in parallel with the progression of bone destruction. The Subcutaneous administration of fentanyl (0.025-0.64 mg/kg), sufentanil (0.005-0.04 mg/kg), and morphine (2.5-40 mg/kg) oil the test days 15 and 22 post-inoculation reduced pain-related behaviors in a dose dependent manner. A complete relief from pain-related behaviors was achieved with the following doses: >= 0. 16 mg/kg fentanyl, 0.02 mg/kg sufentanil, and 20 mg/kg morphine. In conclusion, the results showed a clear link between tumor growth-induced bone destruction and behavioral pain manifestations, the latter was effectively controlled by the opioids fentanyl, sufentanil, and morphine. (c) 2005 Elsevier Inc. All rights reserved.