VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress.
VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress.
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DOI：
10.1016/j.bbagen.2012.06.019
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发表时间：
2012-10
期刊：
影响因子：
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通讯作者：
T. T. Le-T.;K. Mawatari;Miki Maetani;Tomomi Yamamoto;S. Hayashida;Hitomi Iba;Mutsumi Aihara;A. Hirata;T. Shimohata;Takashi Uebanso;A. Takahashi
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文献类型：
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作者：
T. T. Le-T.;K. Mawatari;Miki Maetani;Tomomi Yamamoto;S. Hayashida;Hitomi Iba;Mutsumi Aihara;A. Hirata;T. Shimohata;Takashi Uebanso;A. Takahashi
研究背景活性氧（reactive oxygen species，ROS）包括超氧阴离子自由基（superoxideanion radical，O2），对多种细胞大分子具有慢性氧化损伤的危险性，导致细胞损伤。超氧化物歧化酶（SOD）催化超氧化物歧化为氧和过氧化氢，并且是对抗ROS的主要防御。副溶血性弧菌是一种海洋细菌，在食用生的或未煮熟的海鲜后引起急性胃肠炎，它可以在肠道炎症细胞产生的ROS中存活。然而，关于副溶血性弧菌SOD的信息很少。本研究旨在阐明副溶血性弧菌SOD对ROS. METHODSV的作用。通过GFP报告基因测定来测量副溶血性弧菌SOD基因启动子活性。构建了副溶血性弧菌SOD基因突变体，测定了其SOD活性和抗氧化能力，经SDS生物信息学分析表明，副溶血性弧菌SOD基因突变体的金属辅因子FeSOD（VP 2118）、MnSOD（VP 2860）和CuZnSOD（VPA 1514）具有明显的差异。VP 2118基因启动子活性显著高于其他SOD基因。在VP 2118基因缺失突变体中，SOD活性显著降低，并可通过VP 2118基因互补恢复。VP 2118的缺失导致对过氧化氢、次黄嘌呤-黄嘌呤氧化酶或百草枯产生的ROS的抗性显著降低。结论VP 2118是副溶血弧菌的主要SOD，在抗氧化应激反应中起重要作用。
BACKGROUNDReactive oxygen species (ROS), including superoxide anion radical, induce chronic risk of oxidative damage to many cellular macromolecules resulting in damage to cells. Superoxide dismutases (SODs) catalyze the dismutation of superoxide to oxygen and hydrogen peroxide and are a primary defense against ROS. Vibrio parahaemolyticus, a marine bacterium that causes acute gastroenteritis following consumption of raw or undercooked seafood, can survive ROS generated by intestinal inflammatory cells. However, there is little information concerning SODs in V. parahaemolyticus. This study aims to clarify the role of V. parahaemolyticus SODs against ROS.METHODSV. parahaemolyticus SOD gene promoter activities were measured by a GFP reporter assay. Mutants of V. parahaemolyticus SOD genes were constructed and their SOD activity and resistance to oxidative stresses were measured.RESULTSBioinformatic analysis showed that V. parahaemolyticus SODs were distinguished by their metal cofactors, FeSOD (VP2118), MnSOD (VP2860), and CuZnSOD (VPA1514). VP2118 gene promoter activity was significantly higher than the other SOD genes. In a VP2118 gene deletion mutant, SOD activity was significantly decreased and could be recovered by VP2118 gene complementation. The absence of VP2118 resulted in significantly lowered resistance to ROS generated by hydrogen peroxide, hypoxanthine–xanthine oxidase, or Paraquat. Furthermore, both the N- and C-terminal SOD domains of VP2118 were necessary for ROS resistance.CONCLUSIONVP2118 is the primary V. parahaemolyticus SOD and is vital for anti-oxidative stress responses.GENERAL SIGNIFICANCEThe V. parahaemolyticus FeSOD VP2118 may enhance ROS resistance and could promote its survival in the intestinal tract to facilitate host tissue infection.