Differential effects on HIV-1 gene regulation by EBV in T lymphocytic and promonocytic cells transduced to express recombinant human CR2.
Differential effects on HIV-1 gene regulation by EBV in T lymphocytic and promonocytic cells transduced to express recombinant human CR2.
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DOI：
10.1006/viro.1997.8764
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发表时间：
1997-10
期刊：
影响因子：
3.7
通讯作者：
G. Romano;M. Guan;W. Long;E. Henderson
中科院分区：
文献类型：
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作者：
G. Romano;M. Guan;W. Long;E. Henderson
一组人类造血细胞系通过基因工程表达重组补体受体2 (CR2或CD21)，这也是爱泼斯坦-巴尔病毒（EBV）受体。面板由SupT1、J1.1、U1组成。艾滋病毒细胞。后者是前单核细胞系，而其他两个是T淋巴细胞系。J1.1和U1。HIV细胞被人类免疫缺陷病毒1型（HIV-1）潜伏感染。这三个细胞系用基于小鼠白血病病毒（MLV）的逆转录病毒载体系统转导。CR2在细胞膜上有效且一致地表达，增强了对EBV感染的易感性。重组CR2在造血细胞系中的高效表达，为在合适的细胞培养模型中研究EBV感染与HIV-1基因调控之间的相互作用提供了可能。EBV和HIV-1共感染结果的影响是细胞类型依赖的。在两种T淋巴细胞系中，HIV-1的表达被EBV快速而持续地下调。相反，在单核细胞细胞系U1。HIV-CR2、HIV-1的表达被EBV短暂增强。EBV和HIV-1合并感染导致U1。HIV-CR2细胞具有潜在的重要性，因为在单核细胞样细胞中激活HIV-1基因表达可能在CD4+ T细胞凋亡耗竭的机制中发挥关键作用。因此U1。HIV-CR2细胞系可能为研究EBV和HIV-1在诱导原代CD4+ T细胞凋亡中的协同作用提供了一个有用的细胞培养系统。
A panel of human hematopoietic cell lines was genetically engineered to express recombinant complement receptor 2 (CR2 or CD21), which is also the Epstein-Barr virus (EBV) receptor. The panel was composed of SupT1, J1.1, and U1.HIV cells. The latter is a promonocytic cell line, whereas the other two are T lymphocytic cell lines. J1.1 and U1.HIV cells are latently infected by human immunodeficiency virus type 1 (HIV-1). These three cell lines were transduced with a murine leukemia virus (MLV)-based retroviral vector system. CR2 was efficiently and consistently expressed on the cell membranes, conferring enhanced susceptibility to EBV infection. The efficient expression of recombinant CR2 in cell lines of hematopoietic origin allowed for study of the interaction between EBV infection and HIV-1 gene regulation in suitable cell-culture models. The effects of EBV and HIV-1 coinfection results were cell-type dependent. In the two T lymphocytic cell lines, HIV-1 expression was rapidly and persistently down-regulated by EBV. Conversely, in the promonocytic cell line U1.HIV-CR2, HIV-1 expression was transiently enhanced by EBV. The EBV and HIV-1 coinfection result in U1.HIV-CR2 cells is potentially important, as the activation of HIV-1 gene expression in monocyte-like cells may play a crucial role in the mechanism of CD4+ T cell depletion by apoptosis. Therefore, the U1.HIV-CR2 cell line may represent a useful cell-culture system to study the synergism between EBV and HIV-1 in inducing apoptosis in primary CD4+ T cells.