Isolation and Characterization of Adipose Tissue Macrophages
Isolation and Characterization of Adipose Tissue Macrophages
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DOI：
10.1007/978-1-4939-9195-2_18
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发表时间：
2019-01-01
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影响因子：
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通讯作者：
Roeszer, Tamas
中科院分区：
文献类型：
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作者：
Ampem, Grace;Roeszer, Tamas
本章描述了一种可用于从内脏白色脂肪组织中分离脂肪组织巨噬细胞（ATM）的技术。尽管如此，该技术也可用于从小鼠的皮下白色脂肪组织和棕色脂肪组织、人类皮下脂肪库以及蟾蜍非洲爪蟾的脂肪体中分离ATM。我们详细介绍了流式细胞术的门控策略，已开发出识别ATM人口，我们描述了从这个人口的RNA分离和基因表达谱的使用。最后，我们描述了在体外培养的ATM下游应用。
This chapter describes a technique that can be used to isolate adipose tissue macrophages (ATMs) from the visceral white adipose tissue. Nevertheless, this technique can also be used to isolate ATMs from subcutaneous white adipose tissue and brown adipose tissue from mouse, human subcutaneous fat depot, and also from the fat body of the toad Xenopus. We detail the flow-cytometric gating strategy that has been developed to identify ATM population, and we describe the isolation of RNA from this population and its use for gene expression profiling. Finally, we describe in vitro culture of ATMs for downstream applications.