Imaging axonal transport in the rat visual pathway.
Imaging axonal transport in the rat visual pathway.
复制标题
在大鼠视觉途径中成像轴突传输。
DOI：
10.1364/boe.4.000364
复制
发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Fortune B
中科院分区：
文献类型：
--
作者：
Abbott CJ;Choe TE;Lusardi TA;Burgoyne CF;Wang L;Fortune B
A technique was developed for assaying axonal transport in retinal ganglion cells using 2 µl injections of 1% cholera toxin b-subunit conjugated to AlexaFluor488 (CTB). In vivo retinal and post-mortem brain imaging by confocal scanning laser ophthalmoscopy and post-mortem microscopy were performed. The transport of CTB was sensitive to colchicine, which disrupts axonal microtubules. The bulk rates of transport were determined to be approximately 80–90 mm/day (anterograde) and 160 mm/day (retrograde). Results demonstrate that axonal transport of CTB can be monitored in vivo in the rodent anterior visual pathway, is dependent on intact microtubules, and occurs by active transport mechanisms.
