IDENTIFICATION OF MOSQUITO BLOOD MEALS BY ENZYME-LINKED IMMUNOSORBENT-ASSAY
IDENTIFICATION OF MOSQUITO BLOOD MEALS BY ENZYME-LINKED IMMUNOSORBENT-ASSAY
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DOI：
10.4269/ajtmh.1981.30.1336
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发表时间：
1981-01-01
影响因子：
3.3
通讯作者：
DEFOLIART, GR
中科院分区：
文献类型：
--
作者：
BURKOT, TR;GOODMAN, WG;DEFOLIART, GR
描述了一种用于识别蚊子血粉的微酶联免疫吸附测定法。在兔子和豚鼠中制备用于测定的抗血清，并通过与交叉反应的血清沉淀来纯化。使用少至 58 μg 的摄入血清即可对一般水平进行阳性血源鉴定。正确鉴定了喂食 7 只宿主动物的 38 只实验室蚊子中的 37 只的血粉，其中包括一只含有双重饲料的蚊子。 [测试的宿主动物是红狐 (Vulpes fulva)、灰狐 (Urocyon cinereoargenteus)、狗 (Canis familias)、花栗鼠 (Tamias striatus)、灰松鼠 (Sciurus carolinensis)、条纹地松鼠 (Citellus tridecemlineatus) 和土拨鼠 (Marmota monax)]。
A microenzyme-linked immunosorbent assay for identifying mosquito blood meals is described. Antisera for the assay were prepared in rabbits and guinea pigs and purified by precipitation with cross-reacting blood sera. Positive blood source identifications were made to the generic level using as little as 58 .mu.g of ingested blood serum. The blood meals of 37 of 38 laboratory mosquitoes fed on 7 host animals were correctly identified, including a mosquito that contained a double feed. [Host animals tested were red fox (Vulpes fulva), grey fox (Urocyon cinereoargenteus), dog (Canis familiaris), chipmunk (Tamias striatus), grey squirrel (Sciurus carolinensis), striped ground squirrel (Citellus tridecemlineatus) and woodchuck (Marmota monax).].