The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids
The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids
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DOI：
10.1007/s102380300012
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发表时间：
2003-05-01
影响因子：
4.6
通讯作者：
Miyakawa, I
中科院分区：
文献类型：
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作者：
Kawasaki, F;Kawano, Y;Miyakawa, I
为了研究白细胞介素6（IL-6）和白细胞介素6可溶性受体（sR）在人类排卵中的作用，我们评估了人卵泡液中的浓度，并分析了IL-6和IL-6 sR与卵母细胞成熟的相关性。这些卵母细胞是从 45 名接受体外受精和胚胎移植的女性的卵泡液中获得的。通过ELISA测定卵泡液中IL-6和IL-6 sR的浓度。此外，培养从卵泡液中获得的颗粒细胞，并用毛喉素和12-o-十四烷酰佛波醇13-乙酸酯处理24-48小时。卵泡液中IL-6的浓度显着高于血清中的浓度（P
order to investigate the role of interleukin-6 (IL-6) and interleukin-6 soluble receptor (sR) in human ovulation, we evaluated the concentrations in human follicular fluid and analyzed the correlation of IL-6 and IL-6 sR with oocyte maturation. The oocytes were obtained from the follicular fluid of 45 women undergoing in vitro fertilization and embryo transfer. The concentrations of IL-6 and IL-6 sR in follicular fluid were measured by ELISA. In addition, granulosa cells obtained from the follicular fluid were cultured and treated with forskolin and 12-o-tetradecanoylphorbol 13-acetate for 24-48 h. The concentration of IL-6 was significantly higher in the follicular fluid than in the serum (P