Genetic diversity and population structure of endangered endemic Paeonia jishanensis in China and conservation implications
Genetic diversity and population structure of endangered endemic Paeonia jishanensis in China and conservation implications
复制标题
中国濒危特有吉山芍药遗传多样性、种群结构及保护意义
DOI：
10.1016/j.bse.2016.05.003
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发表时间：
2016-06-01
影响因子：
1.6
通讯作者：
Peng, Li-Ping
中科院分区：
文献类型：
--
作者：
Xu, Xing-Xing;Cheng, Fang-Yun;Peng, Li-Ping
鸡山牡丹是我国特有的观赏和药用植物，是我国栽培牡丹最重要的祖先种之一。利用21个EST-SSR标记对10个居群的236个个体进行了遗传多样性分析。结果表明，居群间遗传多样性水平适中（H-E = 0.340），遗传分化程度较高（F-ST = 0.335）。综合STRUCTURE、PCoA和邻接分析的结果，10个居群可分为4个遗传类群，且与其地理来源有显著相关性，层次AMOVA分析结果进一步支持了这一结论，表明居群间变异最大，达17.9%。Mantel检验表明，居群间遗传距离与地理距离呈显著正相关（r = 0.873，P < 0.0001）。鸡山松的遗传结构可能是由于地理隔离和兼性营养繁殖的繁殖系统阻碍了有限的基因流。该研究对该濒危物种的保护和利用具有重要意义。(C)2016爱思唯尔有限公司版权所有
Paeonia jishanensis, one of the most important ancestral species of cultivated tree peonies, is an endangered ornamental and medicinal plant endemic to China. A total of 236 individuals of P. jishanensis from 10 extant populations were analysed using 21 EST-SSR markers to assess their genetic diversity and population structure. Moderate genetic diversity levels (H-E = 0.340) and high genetic differentiation among populations (F-ST = 0.335) were revealed. Combining the results of STRUCTURE, PCoA and neighbour joining analyses, the 10 populations were divided into four genetic groups that were significantly related to their geographical origins, which was further supported by hierarchical AMOVA showing the highest variation of 17.9% among groups. The Mantel test showed a significant positive correlation between genetic distance and geographic distance among populations (r = 0.873, P < 0.0001). The genetic structure of P. jishanensis may be due to limited gene flow hindered by vicariance and its breeding system: facultative vegetative reproduction. This study carries significant implications for the conservation and utilization of this endangered species. (C) 2016 Elsevier Ltd. All rights reserved.