Evidence from peptidomic analysis of skin secretions that the red-legged frogs, Rana aurora draytonii and Rana aurora aurora, are distinct species
Evidence from peptidomic analysis of skin secretions that the red-legged frogs, Rana aurora draytonii and Rana aurora aurora, are distinct species
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DOI：
10.1016/j.peptides.2005.10.018
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发表时间：
2006-06-01
期刊：
影响因子：
3
通讯作者：
Davidson, Carlos
中科院分区：
文献类型：
--
作者：
Conlon, J. Michael;Al-Ghafari, Nadia;Davidson, Carlos
北方红腿蛙和加州红腿蛙传统上被归为同一物种组。从去甲肾上腺素刺激的红毛酵母皮肤分泌物中分离得到10种具有抗菌活性的多肽。aurora draytonii和纯化到接近同质。这些肽被鉴定为属于ranatuerin-2家族（两种肽）、brevinin-1家族（四种肽）、temporin家族（三种肽）和一种新的肽RV-23（RIGVLLARLPKLFSLFKLMGKKV），其与蜂毒肽蜂毒肽具有有限的结构相似性。这种肽的分布与以前在R.在相同条件下和一年中相同时间收集的Aurora Aurora（一种Ranatuerin-2肽、两种brevinin-1肽和一种temporin肽）。相应的R. aurora draytonii和R. aurora aurora肽与来自美洲组的新世界蛙类的其他成员（波伊氏蛙、白背蛙和黄腹蛙）的直系同源物的序列变化相当。因此，有人建议应将红腿蛙视为单独的物种（R。aurora和R. draytonii）在Amerana组而不是同种亚种。这些数据强调，皮肤分泌物中的抗菌肽的氨基酸序列可以用来推断蛙类物种之间的分类和系统发育关系。(c)2005年由Elsevier Inc.出版
The northern red-legged frog Rana aurora aurora and the California red-legged frog Rana aurora draytonii are traditionally classified together in the same species group. Ten peptides with antimicrobial activity were isolated from norepinephrine-stimulated skin secretions of R. aurora draytonii and purified to near homogeneity. The peptides were identified as belonging to the ranatuerin-2 family (two peptides), brevinin-1 family (four peptides), temporin family (three peptides), and a novel peptide, RV-23 (RIGVLLARLPKLFSLFKLMGKKV) that has limited structural similarity to the bee venom peptide, melittin. This distribution of peptides contrasts with that found previously in skin secretions from R. aurora aurora collected under the same conditions and at the same time of year (one ranatuerin-2 peptide, two brevinin-1 peptides, and one temporin peptide). The variation in amino acid sequences between corresponding R. aurora draytonii and R. aurora aurora peptides is comparable with the variation in sequences of orthologs; from other members of the Amerana group of New World ranid frogs (Rana boylii, Rana muscosa, and Rana luteiventris). It is proposed, therefore, that the red-legged frogs should be regarded as separate species (R. aurora and R. draytonii) within the Amerana group rather than conspecific subspecies. The data emphasize that amino acid sequences of antimicrobial peptides in skin secretions may be used to infer taxonomic and phylogenetic relationships between species of ranid frogs. (c) 2005 Published by Elsevier Inc.