Intraoperative Near-infrared Fluorescence Imaging with Novel Indocyanine Green-Loaded Nanocarrier for Spinal Metastasis: A Preliminary Animal Study.
Intraoperative Near-infrared Fluorescence Imaging with Novel Indocyanine Green-Loaded Nanocarrier for Spinal Metastasis: A Preliminary Animal Study.
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DOI：
10.2174/1874120701206010080
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Ochiai, Naoyuki
中科院分区：
文献类型：
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作者：
Funayama, Toru;Sakane, Masataka;Ochiai, Naoyuki
在脊柱转移瘤切除过程中，边缘切除通常是困难的，因为存在重要的组织，如主动脉、腔静脉和硬脑膜，包括毗邻椎体的脊髓。因此，迫切需要能够清楚地识别骨转移的新术中成像方法。我们提出了一种新型的负载吲哚青绿(ICG)的纳米载体(ICG-乳糖体)，由于其增强的渗透和保留(EPR)作用，具有肿瘤选择性。我们研究术中应用ICG-乳糖体近红外(NIR)荧光诊断脊柱转移瘤的可行性。采用大鼠皮下乳腺肿瘤模型和大鼠乳腺癌脊椎转移模型。皮下肿瘤和脊柱转移瘤发出的荧光在至少24小时内清晰可见。此外，解剖的脊柱成像显示L6椎体转移灶发出清晰的荧光，而正常骨缺乏荧光。本研究是首次报道脊柱转移瘤的近红外荧光成像。ICG-乳糖体近红外荧光成像是一种有效的术中影像检查方法。
Marginal resection during resection of a spinal metastasis is frequently difficult because of the presence of important tissues such as the aorta, vena cava, and dura mater, including the spinal cord adjacent to the vertebral body. Thus, there is an urgent need for novel intraoperative imaging modalities with the ability to clearly identify bone metastasis. We have proposed a novel nanocarrier loaded with indocyanine green (ICG) (ICG-lactosome) with tumor selectivity attributable to its enhanced permeation and retention (EPR) effect. We studied its feasibility in intraoperative near-infrared (NIR) fluorescence diagnosis with ICG-lactosome for imaging spinal metastasis. A rat model of subcutaneous mammary tumor and a rat model of spinal metastasis of breast cancer were used. Fluorescence emitted by the subcutaneous tumors and the spinal metastasis were clearly detected for at least 24 h. Moreover, imaging of the dissected spine revealed clear fluorescence emitted by the metastatic lesion in the L6 vertebra while the normal bone lacked fluorescence. This study was the first report on NIR fluorescence imaging of spinal metastasis in vivo. NIR fluorescence imaging with ICG-lactosome could be an effective intraoperative imaging modality for detecting spinal metastasis.