An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus.
An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus.
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DOI：
10.1016/j.virol.2010.03.013
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发表时间：
2010-06-20
期刊：
影响因子：
3.7
通讯作者：
Goodin M
中科院分区：
文献类型：
--
作者：
Bandyopadhyay A;Kopperud K;Anderson G;Martin K;Goodin M
马铃薯黄矮病毒（PYDV;核弹状病毒类型种）的基因组被确定为12，875个核苷酸（nt）。反基因组由7个开放阅读框（ORF）组成，顺序为3′-N-X-P-Y-M-G-L-5′，它们可能分别编码核衣壳蛋白、磷酸蛋白、运动蛋白、基质蛋白、糖蛋白和RNA依赖性RNA聚合酶蛋白，但X的功能尚不清楚。ORF的3′端为149 nt的前导RNA，5′端为97 nt的尾随RNA，并被保守的基因间连接所分隔。系统发育分析表明，PYDV与其它叶蝉传弹状病毒关系密切。使用功能蛋白测定来确定PYDV蛋白的亚细胞定位。令人惊讶的是，M蛋白能够在没有任何其他病毒蛋白的情况下诱导内核膜的核内积累。最后，双分子荧光互补被用来产生最全面的蛋白质相互作用的地图，植物适应弹状病毒的日期。
The genome of Potato yellow dwarf virus (PYDV; Nucleorhabdovirus type species), was determined to be 12,875 nucleotides (nt). The antigenome is organized into seven open reading frames (ORFs) ordered 3′-N-X-P-Y-M-G-L-5′, which likely encode the nucleocapsid, phospho, movement, matrix, glyco and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. The ORFs are flanked by a 3′ leader RNA of 149 nt and a 5′ trailer RNA of 97 nt, and are separated by conserved intergenic junctions. Phylogenetic analyses indicated that PYDV is closely related to other leafhopper-transmitted rhabdoviruses. Functional protein assays were used to determine the subcellular localization of PYDV proteins. Surprisingly, the M protein was able to induce the intranuclear accumulation of the inner nuclear membrane in the absence of any other viral protein. Finally, bimolecular fluorescence complementation was used to generate the most comprehensive protein interaction map for a plant-adapted rhabdovirus to date.