An unusual arrangement of 13 zinc fingers in the vertebrate gene Z13.
An unusual arrangement of 13 zinc fingers in the vertebrate gene Z13.
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脊椎动物基因 Z13 中 13 个锌指的不寻常排列。
DOI：
10.1042/bj3110219
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. Wells
中科院分区：
文献类型：
--
作者：
T. Schulz;B. Hopwood;P. Rathjen;J. Wells
锌指是一种蛋白质结构域，赋予广泛的功能重要的蛋白质特异性核酸结合活性。在本文中，我们报告了一个新的小鼠锌指基因，mZ 13的分子克隆和鉴定。对mZ 13 cDNA的分析表明，该基因表达由2.7 kb转录物编码的794个氨基酸的蛋白。这种蛋白质有一个不寻常的安排，13个锌指成一个“手”的12个串联的手指和一个单一的孤立的手指附近的C-末端。这种结构组织与可能的鸡同源物cZ 13是保守的。mZ 13在N-末端还含有一个额外的结构域，该结构域先前已通过蛋白质-蛋白质相互作用参与锌指转录因子DNA结合的调节。在广泛的小鼠胚胎和成体组织中检测到mZ 13表达。mZ 13的结构组织及其表达谱表明，它可能作为一个管家DNA结合蛋白，调节特定基因的表达。
The zinc finger is a protein domain that imparts specific nucleic acid-binding activity on a wide range of functionally important proteins. In this paper we report the molecular cloning and characterization of a novel murine zinc-finger gene, mZ13. Analysis of mZ13 cDNAs revealed that the gene expresses a 794-amino-acid protein encoded by a 2.7 kb transcript. The protein has an unusual arrangement of 13 zinc fingers into a 'hand' of 12 tandem fingers and a single isolated finger near the C-terminus. This structural organization is conserved with the probable chicken homologue, cZ13. mZ13 also contained an additional domain at the N-terminus which has previously been implicated in the regulation of zinc-finger transcription factor DNA-binding, via protein-protein interactions. mZ13 expression was detected in a wide range of murine embryonic and adult tissues. The structural organization of mZ13 and its expression profile suggest that it may function as a housekeeping DNA-binding protein that regulates the expression of specific genes.