Visualizing the molecular sociology at the HeLa cell nuclear periphery
Visualizing the molecular sociology at the HeLa cell nuclear periphery
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DOI：
10.1126/science.aad8857
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发表时间：
2016-02-26
期刊：
影响因子：
56.9
通讯作者：
Baumeister, Wolfgang
中科院分区：
文献类型：
--
作者：
Mahamid, Julia;Pfeffer, Stefan;Baumeister, Wolfgang
真核细胞核体积的分子组织在很大程度上仍未被探索。在此，我们结合冷冻电子断层扫描（cryo - ET）的最新进展，制作了海拉（HeLa）细胞核周边的三维快照。核糖体的亚断层平均和分类揭示了细胞质翻译机制的天然结构和组织。对一个大型动态结构——核孔复合体的分析，揭示了在单个复合体层面可检测到的变异。冷冻电子断层扫描被用于原位观察以前难以捉摸的结构，如核小体链和核纤层的细丝。对核纤层结构的阐明有助于深入了解它对后生动物细胞核硬度的贡献。
The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure-the nuclear pore complex-revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness.