Immunochemical approaches to studies of CYP1A localization and induction by xenobiotics in fish.
Immunochemical approaches to studies of CYP1A localization and induction by xenobiotics in fish.
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DOI：
10.1007/978-3-0348-8853-0_5
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Anders Goksøyr;A. Husøy
中科院分区：
文献类型：
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作者：
Anders Goksøyr;A. Husøy
人们越来越认识到，水生环境中的多核芳烃（PAHs）和有机氯化合物（如多氯联苯（PCBs）、某些农药和二恶英）可能导致生理和病理影响，如免疫紊乱、对生殖和发育的影响，甚至肿瘤。接触污染物可能对生物组织的各个层面产生影响，从细胞层面对个体有机体、种群的影响到整个生态系统。细胞色素P450系统（CYP或P450）在内源性和外源性化合物的生物转化中具有重要作用。事实上，许多不同的环境污染物诱导鱼类细胞色素P450 lA （CYP1A）蛋白的新合成，使这些酶在水生毒理学中具有有趣的地位。在过去的二十年中，许多关于鱼类CYP1A系统的研究表明，它作为水生污染的生物标志物是有用的。关于细胞色素P450系统的生化和毒理学方面的一般概述将在这里给出，然后是CYP1A在鱼类中的诱导反应的更详细的描述。将讨论CYP1A诱导的生态毒理学后果和使用免疫化学技术检测CYP1A作为环境监测中的生物标志物。
There is an increasing understanding that polynuclear aromatic hydrocarbons (PAHs) and organochlorine compounds (like polychlorinated biphenyls (PCBs), certain pesticides and dioxins) in the aquatic environment may lead to physiological and pathological effects such as immunological disturbances, effects on reproduction and development, and even neoplasms. Exposure to pollutants may have consequences at all levels in the biological organization, from the cellular level over effects on the individual organism, population, to the entire ecosystem. The cytochrome P450 system (CYP or P450) has an essential function in the biotransformation of endogenous and exogenous compounds. The fact that many different environmental pollutants inducede novosynthesis of cytochrome P450 lA (CYP1A) proteins in fish, gives these enzymes an interesting position in aquatic toxicology. Many investigations concerning the CYP1A system in fish have been performed over the last two decades, demonstrating its usefulness as a biomarker for aquatic pollution. A general overview of the biochemical and toxicological aspects concerning the cytochrome P450 system will be given here, followed by a more detailed description of CYP1A induction responses in fish. Ecotoxicological consequences of CYP1A induction and the use of immunochemical techniques for CYP1A detection as a biomarker in environmental monitoring will be discussed.