Expression and role of SIRT1 in hepatocellular carcinoma
Expression and role of SIRT1 in hepatocellular carcinoma
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DOI：
10.3892/or.2011.1301
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发表时间：
2011-08-01
期刊：
影响因子：
4.2
通讯作者：
Moon, Woo Sung
中科院分区：
文献类型：
--
作者：
Choi, Ha Na;Bae, Jun Sang;Moon, Woo Sung
沉默交配型信息调节2同源物1（SIRT1）是一种多方面的，烟酰胺腺嘌呤二核苷酸依赖性蛋白脱乙酰酶，参与从癌症到衰老的各种细胞过程。检测了90例肝细胞癌（HCC）和5株HCC细胞系中SIRT 1的表达。同时检测10例新鲜肝癌组织中p53基因突变与SIRT1表达的关系。使用合成的小干扰RNA通过RNA干扰（RNAi）沉默SIRT1基因表达，并评估细胞生长和细胞周期进展。SIRT1在肝癌组织中的表达显著高于非肿瘤组织（p
Silent mating type information regulation 2 homolog 1 (SIRT1) is a multifaceted, nicotinamide adenine dinucleotide-dependent protein deacetylase with involvement in a wide variety of cellular processes ranging from cancer to aging. Expression of SIRT1 was evaluated in 90 cases of hepatocellular carcinoma (HCC) and five HCC cell lines. The relationship between the mutation status of p53 and expression of SIRT1 was also investigated in 10 fresh HCC tissues. Synthetic small interfering RNA was used to silence SIRT1 gene expression by RNA interference (RNAi), and cell growth and cell cycle progression were assessed. Expression of SIRT1 was significantly elevated in the HCC tissues when compared to that of non-tumor tissues (p