The effect of EGb-761 on morphologic vasospasm in canine basilar artery after subarachnoid hemorrhage
The effect of EGb-761 on morphologic vasospasm in canine basilar artery after subarachnoid hemorrhage
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DOI：
10.1097/00005344-200309000-00011
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发表时间：
2003-09-01
影响因子：
3
通讯作者：
Üstün, H
中科院分区：
文献类型：
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作者：
Bayar, MA;Erdem, Y;Üstün, H
本研究探讨银杏叶提取物（EGb-761），一种抗氧化剂和血小板活化因子拮抗剂，对实验性犬蛛网膜下腔出血模型的基底动脉血管痉挛的影响。进行形态学分析，并通过放射免疫法测定血清和脑脊液内皮素-1水平。在处理组和未处理组之间进行比较。将24只杂种犬随机分为3组。第1组动物（n = 8）未发生蛛网膜下腔出血，也未接受任何治疗。在该组中，每天测量血清和脑脊液内皮素-1水平，持续8天。在第9天，处死动物并切除其基底动脉进行组织病理学检查。在第2组（n = 8）中，使用自体动脉血产生蛛网膜下腔出血，并在接下来的8天内每天静脉注射生理盐水。如第1组所述评估内皮素-1水平和基底动脉。在第3组（n = 8）中，使用自体动脉血产生蛛网膜下腔出血，每天静脉推注EGb-761，持续8天。如上所述评估内皮素-1水平和基底动脉。比较各组的血清和脑脊液内皮素-1和组织病理学检查结果，I组在8天内血清和脑脊液内皮素-1水平无明显变化，基底动脉组织病理学检查未见异常。在第2组中，血清和脑脊液内皮素-1水平在第2天突然显著升高，并保持高水平至研究期结束（第8天）。组织学检查显示明显的血管痉挛。在第3组中，血清和脑脊液内皮素-1水平与第2组中观察到的模式相同;然而，动脉显示的血管痉挛明显少于第2组中观察到的情况。研究结果未提供血小板活化因子拮抗剂EGb-761作用机制的信息，但明确表明该药物可减轻犬基底动脉中的形态学血管痉挛。
This study investigated the effects of Ginkgo biloba extract (EGb-761), an anti-oxidant and platelet-activating factor antagonist, on basilar artery vasospasm in an experimental canine subarachnoid hemorrhage model. Morphometric analyses were performed, and serum and cerebrospinal fluid endothelin-1 levels were measured by radioimmunoassay. Comparisons were made between treated and untreated groups. Twenty-four mongrel dogs were randomly assigned to three groups. The animals in group 1 (n = 8) were not subjected to subarachnoid hemorrhage and received no treatment. In this group, serum and cerebrospinal fluid endothelin-1 levels were measured daily for 8 days. On day 9, the animals were killed and their basilar arteries were excised for histopathological examination. In group 2 (n = 8), subarachnoid hemorrhage was produced using autologous arterial blood, and daily intravenous boluses of saline were administered for the next 8 days. Assessments of endothelin-1 levels and the basilar arteries were performed as described for group 1. In group 3 (n = 8), subarachnoid hemorrhage was produced using autologous arterial blood, and daily intravenous boluses of EGb-761 were administered for 8 days. Endothelin-1 levels and the basilar arteries were assessed as described above. The groups' serum endothelin-1, cerebrospinal fluid endothelin-1, and histopathological findings were compared.In group I, the serum and cerebrospinal fluid endothelin-1 levels did not change significantly over the 8 days, and histopathological examination of the basilar arteries revealed no abnormalities. In group 2, the serum and cerebrospinal fluid endothelin-1 levels increased abruptly and significantly on day 2, and remained high to the end of the study period (day 8). Histopathological examination revealed marked vasospasm. In group 3, the serum and cerebrospinal fluid endothelin-1 levels followed the same pattern observed in group 2; however, the arteries showed significantly less vasospasm than that observed in group 2.The study findings did not provide information about the mechanism of action of the platelet-activating factor-antagonist EGb-761, but they clearly show that this agent decreases morphologic vasospasm in the dog basilar artery.