Synaptic connectivity between cricket auditory interneurons as studied by selective photoinactivation
Synaptic connectivity between cricket auditory interneurons as studied by selective photoinactivation
复制标题
通过选择性光失活研究蟋蟀听觉中间神经元之间的突触连接
DOI：
10.1523/jneurosci.05-05-01283.1985
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
F. Huber
中科院分区：
文献类型：
--
作者：
A. Selverston;H. Kleindienst;F. Huber
声音检测和定位对蟋蟀很重要。位于前胸神经节的中间神经元在听觉输入的初始处理中起着至关重要的作用。蟋蟀前胸神经节中最容易记录和染色标记的两个听觉中间神经元是欧米茄细胞（ON/1）和上升器（AN/2）。通过一种新的光失活技术选择性地使这些细胞失活，可以研究它们之间的突触关系。我们的结果表明，ON/1细胞相互连接的相互抑制性突触。ON/1细胞对对侧刺激的反应是由另一个ON/1细胞介导的强烈抑制。当一个细胞被杀死时，这种抑制作用被消除，弱的兴奋性反应被揭开。与ON/1细胞不同，AN/2在细胞体侧受到同侧刺激时产生抑制反应，并且当同侧ON/1细胞被杀死时，该反应也被消除，这提供了ON/1是抑制源的强有力证据。与ON/1一样，抑制被弱兴奋取代。
Sound detection and localization are important for crickets. Interneurons located in the prothoracic ganglion play a crucial role in the initial processing of the auditory inputs. Two of the most readily recorded and dye-marked auditory interneurons in the prothoracic ganglion of the cricket are the omega cells (ON/1) and the ascenders (AN/2). By using a new photoinactivation technique to selectively inactivate these cells, the synaptic relationship between them could be studied. Our results indicate that the ON/1 cells are connected to each other with reciprocal inhibitory synapses. An ON/1 cell responds to contralateral stimulation with strong inhibition mediated by the other ON/1 cell. When one cell is killed, this inhibition is removed, and a weak excitatory response is unmasked. Unlike the ON/1 cell, AN/2 produces an inhibitory response when stimulated ipsilaterally to the cell body side, and this response is also removed when the ipsilateral ON/1 cell is killed, providing strong evidence that ON/1 is the source of the inhibition. As with the ON/1, the inhibition is replaced by weak excitation.