Transcriptome profiling based on larvae at different time points after hatching provides a core set of gene resource for understanding the immune response mechanisms of the egg-protecting behavior against Vibrio anguillarum infection in Amphioctopus fangsiao
Transcriptome profiling based on larvae at different time points after hatching provides a core set of gene resource for understanding the immune response mechanisms of the egg-protecting behavior against Vibrio anguillarum infection in Amphioctopus fangsiao
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DOI：
10.1016/j.fsi.2022.04.030
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发表时间：
2022-04-26
影响因子：
4.7
通讯作者：
Li, Zan
中科院分区：
文献类型：
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作者：
Bao, Xiaokai;Wang, Weijun;Li, Zan
软体动物因其独特的免疫系统而受到越来越多的关注。方肖角章鱼等软体动物是经济上重要的头足类动物，其卵保护行为对幼虫免疫反应的影响尚不清楚。同时，对鳃弧菌等致病菌感染头足类幼虫的耐药反应研究较少。本研究以不同护卵行为下的鳗纹弧菌感染初孵的方小斑拟蚊幼虫24 h，通过转录组分析，共鉴定出7156个差异表达基因（deg）。GO和KEGG富集分析显示，多个免疫相关的GO术语和KEGG信号通路富集。蛋白质-蛋白质相互作用网络（PPI网络）用于搜索免疫相关deg之间的功能关系。最后，共鉴定出20个与多种基因功能相关或参与多种信号通路的枢纽基因，并利用定量RT-PCR验证其准确性。首次采用PPI网络研究了不同护卵行为对鳗弧菌侵染后方小姬蚊幼虫的影响。该结果为探索无脊椎动物幼虫免疫过程提供了重要的遗传资源。该数据为进一步研究无脊椎动物感染后的免疫应答机制奠定了基础。
Mollusks have recently received increasing attention because of their unique immune systems. Mollusks such as Amphioctopus fangsiao are economically important cephalopods, and the effects of their egg-protecting behavior on the larval immune response are unclear. Meanwhile, little research has been done on the resistance response of cephalopod larvae infected with pathogenic bacteria such as Vibrio anguillarum. In this study, V. anguillarum was used to infect the primary hatching A. fangsiao larvae under different egg-protecting behaviors for 24 h, and a total of 7156 differentially expressed genes (DEGs) were identified at four time points after hatching based on transcriptome analysis. GO and KEGG enrichment analyses showed that multiple immune-related GO terms and KEGG signaling pathways were enriched. Protein-protein interaction networks (PPI networks) were used to search functional relationships between immune-related DEGs. Finally, 20 hub genes related to multiple gene functions or involved in multiple signaling pathways were identified, and their accuracy was verified using quantitative RT-PCR. PPI networks were first used to study the effects A. fangsiao larvae after infection with V. anguillarum under different egg-protecting behaviors. The results provide significant genetic resources for exploring invertebrate larval immune processes. The data lays a foundation for further study the immune response mechanisms for invertebrates after infection.