Trimethyltin chloride (TMT) neurobehavioral toxicity in embryonic zebrafish.
Trimethyltin chloride (TMT) neurobehavioral toxicity in embryonic zebrafish.
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DOI：
10.1016/j.ntt.2011.09.003
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发表时间：
2011-11
影响因子：
2.9
通讯作者：
Dong Q
中科院分区：
文献类型：
--
作者：
Chen J;Huang C;Zheng L;Simonich M;Bai C;Tanguay R;Dong Q
氯化三甲基锡（TMT）是一种神经毒性物质，广泛存在于水生环境中，主要来自PVC塑料的生产，但很少有研究评估水生神经毒性。我们研究了TMT剂量依赖性畸形和神经行为毒性的胚胎斑马鱼模型。胚胎在受精后48-72小时（hpf）暴露于TMT（0-10 μM），导致畸形发生率呈浓度相关性增加（0-100%），EC 25为5.55 μM。TMT还显着调制的频率，尾巴弯曲，最早的运动行为中观察到的发展斑马鱼，并能够响应机械尾巴触摸。从48-72 hpf暴露于5 μM TMT调节受精后4和5天的光运动反应，并显著促进尾部的凋亡。我们的研究证明了发育中的斑马鱼对TMT的形态和行为敏感性，并为未来识别TMT毒性的受影响途径和化学调节剂建立了平台。
Trimethyltin chloride (TMT) is a neurotoxicant that is widely present in the aquatic environment, primarily from the manufacture of PVC plastic, but few studies have evaluated aquatic neurotoxicity. We have examined TMT dose-dependent malformation and neurobehavioral toxicity in the embryonic zebrafish model. Exposure of embryos to TMT (0–10 μM) from 48–72 hours post fertilization (hpf) elicited a concentration-related increase (0–100%) in malformation incidence with an EC25 of 5.55 μM. TMT also significantly modulated the frequency of tail flexion, the earliest motor behavior observed in developing zebrafish, and the ability to respond to a mechanical tail touch. Exposure to 5 μM TMT from 48–72 hpf modulated the photomotor response at 4 and 5 days post fertilization and significantly promoted apoptosis in the tail. Our study demonstrates the morphological and behavioral sensitivity of the developing zebrafish to TMT and establishes a platform for future identification of the affected pathways and chemical modulators of TMT toxicity.