Study on penetration of titanium dioxide (TiO2) nanoparticles into intact and damaged skin in vitro
Study on penetration of titanium dioxide (TiO2) nanoparticles into intact and damaged skin in vitro
复制标题
DOI：
10.2131/jts.35.107
复制
发表时间：
2010-02-01
影响因子：
2
通讯作者：
Fujii, Makiko
中科院分区：
文献类型：
--
作者：
Senzui, Mika;Tamura, Toshiaki;Fujii, Makiko
定性、定量测定纳米粒在皮肤中的渗透性对纳米粒的毒理学评价具有重要意义。四种不同类型的金红石型二氧化钛（TiO 2）的皮肤渗透性（T-35，35 nm，无涂层; TC-35，35 rim，具有氧化铝/二氧化硅/硅涂层; T-disp，10 x 100 nm，氧化铝涂层和硅涂层颗粒的混合物，分散在环戊二烯基聚硅氧烷中; T-250，250 nm，无涂层）用尤卡坦小型猪的体外完整、剥离和脱毛皮肤测定，以研究分散和皮肤条件的影响。将TiO 2以10%的浓度悬浮在用于化妆品的挥发性硅油环戊硅氧烷中。悬浮液以2 μ l/cm（2）的剂量施用24小时，然后进行氰基丙烯酸酯剥离。用ICP-MS法测定皮肤中的钛浓度，T-35和T-250在悬浮液中易聚集，平均粒径大于1 μ m。TC-35和T-disp显示出良好的分散性能，在悬浮液中的平均直径约为100 nm。在完整和剥离的皮肤中，无论TiO 2类型如何，均未观察到渗透。当TC-35应用于去除毛发的皮肤时，皮肤中的Ti浓度显著较高。SEM-EDS观察表明，Ti渗透到空毛囊（皮肤表面以下大于1 mm），但未渗透到真皮或有活力的表皮。
It is important for toxicological assessment of nanoparticles to determine the penetration of nanoparticle in skin qualitatively and quantitatively. Skin penetration Of four different types of rutile titanium dioxide (TiO2) (T-35, 35 nm, non-coating; TC-35, 35 rim, with almina/silica/silicon coating; T-disp, 10 x 100 nm, mixture of almina coated and silicon coated particles, dispersed in cyclopentasiloxan; T-250, 250 nm, non-coating) was determined with in vitro intact, stripped, and hair-removed skin of Yucatan micropigs to study the effect of dispersion and skin conditions. The TiO2 was suspended in a volatile silicone fluid used for cosmetics, cyclopentasiloxane, at a concentration of 10%. The suspension was applied at a dose 2 mu l/cm(2) for 24 hr, followed by cyanoacrylate stripping. The Ti concentration in skin was determined by ICP-MS. T-35 and T-250 easily aggregated in Suspension with a mean diameter greater than 1 mu m. TC-35 and T-disp showed good dispersion properties with a mean diameter in suspension of approximately 100 nm. No penetration was observed regardless of TiO2 type in intact and stripped skin. The concentration of Ti in skin was significantly higher when TC-35 was applied on hair-removed skin. SEM-EDS observation showed that Ti penetrated into vacant hair follicles (greater than 1 mm below the skin Surface), however, it did not penetrate into dermis or viable epidermis.