HS-SPME-GC-MS analysis of body odor to test the efficacy of foot deodorant formulations
HS-SPME-GC-MS analysis of body odor to test the efficacy of foot deodorant formulations
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DOI：
10.1111/j.1600-0846.2009.00399.x
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发表时间：
2009-11-01
影响因子：
2.2
通讯作者：
Valgimigli, Luca
中科院分区：
文献类型：
--
作者：
Caroprese, Alessandro;Gabbanini, Simone;Valgimigli, Luca
背景/目的脚臭大多是由于细菌代谢产生的短链脂肪酸引起的外分泌出汗。我们的目标是制定一个协议，用GC-MS分析来自六名健康志愿者的脚的目标脂肪酸的顶空固相微萃取。在用生理溶液洗涤脚并在36 ℃下孵育24- 30分钟后，在每个受试者中进行治疗的脚与未治疗的脚的比较分析。72 h胰蛋白酶大豆琼脂生长medium.ResultsAcetic，丁酸，异丁酸和异戊酸被确定为在大多数志愿者的脚恶臭的主要贡献者。丙酸，戊酸和异己酸也检测到一些科目。结论该方法简便、灵敏、无创，可用于足部除臭剂的药效评价。
Background/purposeFoot malodor is mostly due to short-chain fatty acids produced by bacterial metabolism of eccrine sweating. We aimed to develop a protocol for an objective (instrumental) efficacy evaluation of foot deodorant formulations.MethodsHead-space solid-phase microextractions of target fatty acids from the feet of six healthy volunteers were analyzed by GC-MS. A comparative analysis of the treated vs. the untreated foot was performed in each subject after washing the feet with a physiologic solution and incubating at 36 degrees C for 24-72 h in tryptic soy agar growth medium.ResultsAcetic, butyric, isobutyric and isovaleric acids were identified as the main contributors to foot malodor in the majority of volunteers. Propionic, valeric and isocaproic acids were also detected in some subjects. Comparative analysis according to the protocol developed showed a statistically significant (P < 0.01) reduction of target fatty acids ranging from -26.6% to -77.0%.ConclusionThe protocol developed is a convenient, sensitive and non-invasive method to test the efficacy of foot deodorant formulations in human volunteers.