Isolation and identification of a cytopathic activity in Tannerella forsythia
Isolation and identification of a cytopathic activity in Tannerella forsythia
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DOI：
10.1016/j.bbrc.2006.10.012
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发表时间：
2006-12-08
影响因子：
3.1
通讯作者：
Arakawa, Shinichi
中科院分区：
文献类型：
--
作者：
Nakajima, Takuma;Tomi, Naoko;Arakawa, Shinichi
病原体与宿主的相互作用可导致牙周炎、牙齿支持组织的崩解等人类疾病。Tannerella pasthia与牙周炎有关，在细菌中发现了几种细胞病变试剂;然而，其对疾病的贡献仍不清楚。探索细胞病变效应的生物化学方法揭示了T细胞（ATCC 43037）提取物中的两种不同活性;一种使贴壁细胞与基质分离，另一种使细胞停滞在G2。鉴定了前活性的执行者--雷氏菌分离因子（FDF）;其基因组序列和肽酶活性表明FDF是推定的PrtH的实质形式; prtH基因被假设直接从细菌的DNA片段中鉴定，其天然产物从未显示。由于FDF是在细菌培养上清液中发现的，因此其活性意味着对组织的分解有贡献，尽管FDF如何干扰细胞锚定的机制仍然难以捉摸。(c)2006爱思唯尔公司All rights reserved.
Interactions between pathogens and host induce human disorders including periodontitis, disintegration of the tooth supporting tissues. Tannerella forsythia has been linked to the periodontitis and several cytopathic reagents have been found in the bacterium; however, its contribution to the disease remains unclear. Biochemical approach to explore the cytopathic effect revealed two distinct activities in T forsythia (ATCC 43037) extract; one detaches adherent cells from substratum and another arrests cells at G2. An executor of former activity, forsythia detaching factor (FDF) was identified; its genomic sequence and peptidase activity revealed that FDF is a substantial form of putative PrtH; prtH gene was hypothetically identified directly from a DNA fragment of the bacterium and its native product has never been shown. Since FDF was found in the bacterial culture supernatant, its activity implies a contribution to the disintegration of tissues although the mechanism how FDF disturbs cellular anchors remains elusive. (c) 2006 Elsevier Inc. All rights reserved.