The MatP/matS Site-Specific System Organizes the Terminus Region of the E-coli Chromosome into a Macrodomain
The MatP/matS Site-Specific System Organizes the Terminus Region of the E-coli Chromosome into a Macrodomain
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DOI：
10.1016/j.cell.2008.08.031
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发表时间：
2008-10-31
期刊：
影响因子：
64.5
通讯作者：
Espeli, Olivier
中科院分区：
文献类型：
--
作者：
Mercier, Romain;Petit, Marie-Agnes;Espeli, Olivier
将大肠杆菌染色体组织成绝缘大结构域会影响姐妹染色单体的分离和染色体 DNA 的迁移性。在这里，我们报告将末端区 (Ter) 组织成宏结构域依赖于称为 matS 的 13 bp 基序的存在，该基序在 800 kb 长的结构域中重复 23 次。 matS 位点是大肠杆菌染色体中一种新鉴定的名为 MatP 的蛋白质的主要目标。 MatP 在细胞中积累为与 Ter 宏域共定位的离散焦点。 MatP 失活的影响揭示了其作为 Ter 宏结构域主要组织者的作用：在没有 MatP 的情况下，DNA 的压缩程度较低，标记物的移动性增加，并且 Ter 宏结构域的分离发生在细胞周期的早期。我们的结果表明，末端区域需要特定的组织系统来进行细胞周期期间的细菌染色体管理。
The organization of the Escherichia coli chromosome into insulated macrodomains influences the segregation of sister chromatids and the mobility of chromosomal DNA. Here, we report that organization of the Terminus region (Ter) into a macrodomain relies on the presence of a 13 bp motif called matS repeated 23 times in the 800-kb-long domain. matS sites are the main targets in the E. coli chromosome of a newly identified protein designated MatP. MatP accumulates in the cell as a discrete focus that colocalizes with the Ter macrodomain. The effects of MatP inactivation reveal its role as main organizer of the Ter macrodomain: in the absence of MatP, DNA is less compacted, the mobility of markers is increased, and segregation of Ter macrodomain occurs early in the cell cycle. Our results indicate that a specific organizational system is required in the Terminus region for bacterial chromosome management during the cell cycle.