An engineered pathway for the biosynthesis of renewable propane.
An engineered pathway for the biosynthesis of renewable propane.
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DOI：
10.1038/ncomms5731
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发表时间：
2014-09-02
影响因子：
16.6
通讯作者：
Jones, Patrik R.
中科院分区：
文献类型：
--
作者：
Kallio, Pauli;Pasztor, Andras;Thiel, Kati;Akhtar, M. Kalim;Jones, Patrik R.
下一代可再生生物燃料的部署可以通过改善其与现有运输、储存和利用基础设施的兼容性而得到加强。丙烷是液化石油气的主要成分，由于它已经拥有全球市场，因此是一个有吸引力的目标。此外，它在标准条件下是一种气体，但很容易液化。这允许燃料在合成后立即从生物催化过程中分离出来，但不排除作为液体进行能量密集型存储。在这里，我们首次报道了产生可再生丙烷的合成代谢途径。该途径基于丁酰基载体蛋白(ACP)特有的硫代酯酶，该酶允许大肠杆菌宿主的天然脂肪酸生物合成重定向到合成烷烃途径。丙烷的生物合成明显受到电子供给体模块的刺激，从而优化了氧气供应的平衡，并去除了天然的醛还原酶。丙烷是液化石油气的主要成分，具有广泛的商业用途。在这里，作者在大肠杆菌中设计了一条合成代谢途径，并首次展示了丙烷的可再生生产。
The deployment of next-generation renewable biofuels can be enhanced by improving their compatibility with the current infrastructure for transportation, storage and utilization. Propane, the bulk component of liquid petroleum gas, is an appealing target as it already has a global market. In addition, it is a gas under standard conditions, but can easily be liquefied. This allows the fuel to immediately separate from the biocatalytic process after synthesis, yet does not preclude energy-dense storage as a liquid. Here we report, for the first time, a synthetic metabolic pathway for producing renewable propane. The pathway is based on a thioesterase specific for butyryl-acyl carrier protein (ACP), which allows native fatty acid biosynthesis of the Escherichia coli host to be redirected towards a synthetic alkane pathway. Propane biosynthesis is markedly stimulated by the introduction of an electron-donating module, optimizing the balance of O2 supply and removal of native aldehyde reductases. Propane is the main component of liquid petroleum gas and has a wide variety of commercial applications. Here, the authors engineer a synthetic metabolic pathway in E. coli, and demonstrate for the first time the renewable production of propane.
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影响因子：
64.8
作者：
Choi, Yong Jun;Lee, Sang Yup
通讯作者：
Lee, Sang Yup
影响因子：
2.9
作者：
Li, Ning;Chang, Wei-chen;Bollinger, J. Martin, Jr.
通讯作者：
Bollinger, J. Martin, Jr.
DOI：
10.1073/pnas.1200074109
发表时间：
2012-04-17
影响因子：
11.1
作者：
Lan, Ethan I.;Liao, James C.
通讯作者：
Liao, James C.
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
6.4
作者：
Liu, Hui;Yu, Chao;Xian, Mo
通讯作者：
Xian, Mo