Distribution of carcinoembryonic antigen-related cellular adhesion molecules in human gingiva
Distribution of carcinoembryonic antigen-related cellular adhesion molecules in human gingiva
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DOI：
10.1111/j.1600-0722.2012.00981.x
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发表时间：
2012-10-01
影响因子：
1.9
通讯作者：
Blix, Inger J. S.
中科院分区：
文献类型：
--
作者：
Huynh-Torlakovic, Hong;Bjerkan, Louise;Blix, Inger J. S.
癌胚抗原相关细胞粘附分子（CEACAMs）是上皮细胞、内皮细胞、淋巴细胞和骨髓细胞中产生的糖蛋白。癌胚抗原相关细胞粘附分子介导细胞接触和宿主病原体相互作用。本研究的目的是绘制人类牙龈部位CEACAMs的分布图，并研究其调节作用。对牙周炎部位的人类牙龈活检进行的定量实时PCR显示了CEACAM 1，-5，-6和-7的mRNA编码。免疫组化结果显示，除牙周炎组织中CEACAM 5阳性外，牙龈上皮中未见CEACAM 5阳性。癌胚抗原相关的细胞粘附分子1，5和6存在于牙周炎的口腔沟上皮，但在健康牙龈。在结合上皮中，所有三种分子都存在于健康牙龈中，但在牙周炎中仅检测到CEACAM 1和-6。CEACAM 1和-6的染色也见于牙周炎的炎性细胞浸润。促炎介质，包括脂多糖（LPS），肿瘤坏死因子-a（TNF-α）/白细胞介素-1 β（IL-1 β），干扰素-？(IFN-?),增加培养的人口腔角质形成细胞中CEACAM 1和CEACAM 6 mRNA的表达。CEACAM 1和CEACAM 6 mRNA在溶血磷脂酸刺激后也强烈上调。总之，不同CEACAMs的分布与牙龈中的特定位点有关。这可能反映了该组织中的不同功能作用。
Carcinoembryonic antigen-related cellular adhesion molecules (CEACAMs) are glycoproteins produced in epithelial, endothelial, lymphoid, and myeloid cells. Carcinoembryonic antigen-related cellular adhesion molecules mediate cellcell contact and hostpathogen interactions. The aims of this study were to map the distribution and examine the regulation of CEACAMs in human gingival sites. Quantitative real-time PCR performed on human gingival biopsies from periodontitis sites revealed mRNA coding for CEACAM1, -5, -6, and -7. Immunohistochemistry showed that CEACAMs were not found in oral gingival epithelium, except for CEACAM5 in periodontitis. Carcinoembryonic antigen-related cellular adhesion molecules 1, 5, and 6 were present in the oral sulcular epithelium of periodontitis but not in that of healthy gingiva. In junctional epithelium, all three molecules were present in healthy gingiva, but in periodontitis only CEACAM1 and -6 were detected. Staining for CEACAM1 and -6 was also seen in the inflammatory cell infiltrate in periodontitis. No staining for CEACAM7 was found. Proinflammatory mediators, including lipopolysaccharide (LPS), tumour necrosis factor-a (TNF-a)/interleukin-1 beta (IL-1 beta), and interferon-? (IFN-?), increased the expression of CEACAM1 and CEACAM6 mRNAs in cultured human oral keratinocytes. CEACAM1 and CEACAM6 mRNAs were also strongly up-regulated upon stimulation with lysophosphatidic acid. In conclusion, the distribution of different CEACAMs was related to specific sites in the gingiva. This might reflect different functional roles in this tissue.