ANALYSIS OF EPITOPIC RESIDUES INTRODUCED INTO THE HYBRID PEPTIDE VACCINES PREPARED ACCORDING TO THE CASSETTE THEORY
ANALYSIS OF EPITOPIC RESIDUES INTRODUCED INTO THE HYBRID PEPTIDE VACCINES PREPARED ACCORDING TO THE CASSETTE THEORY
复制标题
DOI：
10.1016/0264-410x(94)90285-2
复制
发表时间：
1994-07-01
期刊：
影响因子：
5.5
通讯作者：
ONOE, K
中科院分区：
文献类型：
--
作者：
NARUSE, H;OGASAWARA, K;ONOE, K
在我们之前的研究中，我们通过将血凝素（HA） 127-133引入I-A（b,d）结合肽46F50V54A的43-46和54-58残基组成的I-A（b,d）结合成分，制备了抗流感株a /爱知/2/68 （H3N2）病毒的合成肽疫苗（46F/HA127-133/54A）。该杂交肽疫苗在I-A(b)小鼠中诱导了对A/爱知/2/68和肽疫苗的相当大的免疫应答。在本研究中，我们试图通过将不同长度的HA肽引入46F50V54A的残基43-46和54-58或43-47和53-58组成的I-A（b,d）结合成分中来提高肽疫苗的免疫原性。我们在这里证明，在制备的肽疫苗中，46F/HAI27-133/54A （18 mer）由HA127-133和由46F50V54A的43-47和53-58构建的I-A（b,d）结合成分组成，在I-A(b)和I-A(d)小鼠中诱导最强烈的t细胞反应和中和A/爱知/2/68抗体。
In our previous study, we prepared a synthetic peptide vaccine (46F/HA127-133/54A) against influenza strain A/Aichi/2/68 (H3N2) virus by introducing haemagglutinin (HA) 127-133 to an I-A(b,d) binding component that consisted of residues 43-46 and 54-58 of an I-A(b,d) binding peptide, 46F50V54A. This hybrid peptide vaccine induced considerable immunological responses against A/Aichi/2/68 as well as against the peptide vaccine in I-A(b) mice. In the present study, we have attempted to increase the immunogenicity of the peptide vaccine by introducing HA peptides of various lengths into the I-A(b,d) binding components consisting of residues 43-46 and 54-58 or 43-47 and 53-58 of 46F50V54A. We demonstrate here that, among the peptide vaccines prepared, 46F/HAI27-133/54A (18 mer) consisting of HA127-133 and the I-A(b,d) binding component constructed from 43-47 and 53-58 of 46F50V54A induces the most vigorous T-cell responses and neutralizing antibodies against A/Aichi/2/68 in both I-A(b) and I-A(d) mice.