Mass-spectrometry-based draft of the Arabidopsis proteome
Mass-spectrometry-based draft of the Arabidopsis proteome
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DOI：
10.1038/s41586-020-2094-2
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发表时间：
2020-03-11
期刊：
影响因子：
64.8
通讯作者：
Kuster, Bernhard
中科院分区：
文献类型：
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作者：
Mergner, Julia;Frejno, Martin;Kuster, Bernhard
植物对于生命至关重要，是极其多样化的生物体，具有独特的分子功能(1)。在这里，我们展示了模式植物拟南芥 30 个组织的转录组、蛋白质组和磷酸蛋白质组的定量图谱。我们的分析提供了以下问题的初步答案：有多少基因以蛋白质形式存在（超过 18,000 个）、它们在何处表达、大致数量（动态范围超过六个数量级）以及它们被磷酸化的程度（超过 43,000 个位点）。我们提供了如何使用数据的示例，例如发现从短开放阅读框翻译的蛋白质，揭示参与蛋白质生产调节的序列基序，以及识别组织特异性蛋白质复合物或磷酸化介导的信号事件。 ProteomicsDB 和 ATHENA 数据库为植物界提供了对该资源的交互式访问，其中包括强大的生物信息学工具，用于探索和表征拟南芥蛋白质、其修饰和相互作用。模式植物拟南芥 30 个组织的转录组、蛋白质组和磷酸蛋白质组的定量图谱为植物研究提供了宝贵的资源。
Plants are essential for life and are extremely diverse organisms with unique molecular capabilities(1). Here we present a quantitative atlas of the transcriptomes, proteomes and phosphoproteomes of 30 tissues of the model plant Arabidopsis thaliana. Our analysis provides initial answers to how many genes exist as proteins (more than 18,000), where they are expressed, in which approximate quantities (a dynamic range of more than six orders of magnitude) and to what extent they are phosphorylated (over 43,000 sites). We present examples of how the data may be used, such as to discover proteins that are translated from short open-reading frames, to uncover sequence motifs that are involved in the regulation of protein production, and to identify tissue-specific protein complexes or phosphorylation-mediated signalling events. Interactive access to this resource for the plant community is provided by the ProteomicsDB and ATHENA databases, which include powerful bioinformatics tools to explore and characterize Arabidopsis proteins, their modifications and interactions.A quantitative atlas of the transcriptomes, proteomes and phosphoproteomes of 30 tissues of the model plant Arabidopsis thaliana provides a valuable resource for plant research.