Facile Synthesis of Gd-Cu-In-S/ZnS Bimodal Quantum Dots with Optimized Properties for Tumor Targeted Fluorescence/MR In Vivo Imaging
Facile Synthesis of Gd-Cu-In-S/ZnS Bimodal Quantum Dots with Optimized Properties for Tumor Targeted Fluorescence/MR In Vivo Imaging
复制标题
轻松合成具有优化特性的 Gd-Cu-In-S/ZnS 双峰量子点，用于肿瘤靶向荧光/MR 体内成像
DOI：
10.1021/acsami.5b05372
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发表时间：
2015
影响因子：
9.5
通讯作者：
Chang Jin
中科院分区：
文献类型：
--
作者：
Yang Weitao;Guo Weisheng;Gong Xiaoqun;Zhang Bingbo;Wang Sheng;Chen Na;Yang Wentao;Tu Yu;Fang Xiangming;Chang Jin
双模式成像技术因其在肿瘤早期准确诊断的曙光时代的潜在作用而受到广泛关注。高效低毒、无螯合的健壮双模成像纳米探针对于肿瘤靶向双模活体成像具有重要意义。如何使无镉双峰纳米探针既具有较强的荧光强度，又具有较高的驰豫性能，仍然是一个重要的问题。本论文开发了一种简便的合成方法来制备性能优化的Gd掺杂CuInS/ZnS双峰量子点(GCIS/ZnS，BQds)。通过改变反应温度、陈化时间和硫化锌涂层，可以彻底优化GCIS/ZnS BQD的荧光性能。可以很好地控制Gd前驱体的量，以实现磁流变弛豫率和光学性能之间的优化平衡。用聚乙二醇化的葡聚糖-硬脂酸聚合物脂泡(PEGylated Degan-stearyl acid Poly Lipoices，PEG-DS Plvs)将疏水的GCIS/ZnS BQD表面工程处理到水相中。在相转移后，亲水的GCIS/ZnS@PlVS在水中表现出明显的近红外荧光和高的纵向弛豫度(r1=9.45 mm-1s-1)，具有良好的胶体稳定性。在荷瘤动物实验中，进一步验证了GCIS/ZnS@PlVS可以实现肿瘤靶向的MR/荧光双模成像。在活体实验中未观察到毒性。GCIS/ZnS@Plvs作为肿瘤诊断的双峰造影剂具有很大的潜力。
Dual-modal imaging techniques have gained intense attention for their potential role in the dawning era of tumor early accurate diagnosis. Chelate-free robust dual-modal imaging nanoprobes with high efficiency and low toxicity are of essential importance for tumor targeted dual-modalin vivoimaging. It is still a crucial issue to endow Cd-free dual-modal nanoprobes with bright fluorescence as well as high relaxivity. Herein, a facile synthetic strategy was developed to prepare Gd-doped CuInS/ZnS bimodal quantum dots (GCIS/ZnS, BQDs) with optimized properties. The fluorescent properties of the GCIS/ZnS BQDs can be thoroughly optimized by varying reaction temperature, aging time, and ZnS coating. The amount of Gd precursor can be well-controlled to realize the optimized balance between the MR relaxivity and optical properties. The obtained hydrophobic GCIS/ZnS BQDs were surface engineered into aqueous phase with PEGylated dextran-stearyl acid polymeric lipid vesicles (PEG-DS PLVs). Upon the phase transfer, the hydrophilic GCIS/ZnS@PLVs exhibited pronounced near-infrared fluorescence as well as high longitudinal relaxivity (r1= 9.45 mM–1S–1) in water with good colloidal stability.In vivotumor-bearing animal experiments further verified GCIS/ZnS@PLVs could achieve tumor-targeted MR/fluorescence dual-modal imaging. No toxicity was observed in thein vivoandex vivoexperiments. The GCIS/ZnS@PLVs present great potential as bimodal imaging contrast agents for tumor diagnosis.