Fractionation of rat liver nuclei by sucrose gradient centrifugation.
Fractionation of rat liver nuclei by sucrose gradient centrifugation.
复制标题
通过蔗糖梯度离心分离大鼠肝细胞核。
DOI：
10.1016/0014-4827(68)90187-0
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发表时间：
1968
影响因子：
3.7
通讯作者：
C. Albrecht
中科院分区：
文献类型：
--
作者：
C. Albrecht
本文描述了一种用线性蔗糖梯度和低速离心法部分分离四倍体和二倍体大鼠肝实质和间质细胞核的技术，测定了不同核组分中~（14）C-6-乳清酸的体内掺入，结果表明，二倍体和四倍体实质细胞核具有相同的比放射性，而间质细胞核只有约10%的活性。该技术也可用于确定所研究的肝脏中各种细胞核的百分比。
A technique for the partial fractional separation of tetraploid and diploid parenchymal and stromal rat liver nuclei using a linear, sucrose gradient and low speed centrifugation is described.In vivoincorporation of14C-6-orotic acid in different nuclear fractions was measured and it was shown that the diploid and tetraploid parenchymal nuclei had the same specific radioactivity whereas the stromal nuclei were only about 10 per cent as active. The technique can also be used to determine the percentages of the various kinds of nuclei in the liver being studied.