Validation of a Dehydroepiandrosterone-Sulfate Assay in Three Platyrrhine Primates (Alouatta caraya, Aotus azarae infulatus, and Sapajus apella)
Validation of a Dehydroepiandrosterone-Sulfate Assay in Three Platyrrhine Primates (Alouatta caraya, Aotus azarae infulatus, and Sapajus apella)
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DOI：
10.1007/s10764-021-00239-x
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发表时间：
2021-08-16
影响因子：
2.5
通讯作者：
Takeshita, Rafaela S. C.
中科院分区：
文献类型：
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作者：
da Silva, Gessiane Pereira;de Melo, Josi Teixeira;Takeshita, Rafaela S. C.
脱氢表雄酮（DHEA）及其硫酸酯（DHEAS）是人类和一些非人类灵长类动物中最丰富的循环类固醇，并且与发育、衰老和应激调节有关。我们的目的是验证一种市售的酶免疫测定试剂盒，以测量粪便和血清中的DHEAS的三个阔鼻灵长类动物物种（Alouatta caraya，Aotus azarae infulatus，和Sapajus apella）在圈养。我们从每个物种的一只雄性和一只雌性中收集血清样本。为了验证粪便样本的试剂盒，我们通过对每个物种的一只成年雌性动物口服DHEA进行了生理学验证。我们还测量了四只雌性Alouatta caraya个体在妊娠第三学期和两只雌性产后的粪便DHEAS水平。我们从10个个体中共获得了54个粪便样品和6个血清样品。我们通过在所有种属的粪便和血清提取物中进行平行度和准确度试验，对该测定法进行了分析验证。我们观察到在所有三种动物中口服DHEA后24小时DHEAS的峰值，A. caraya表现出最强的反应和最高的基线浓度。妊娠A. caraya（57，843.86 +/-37，160.31纳克/克），分娩后下降（1，539.07 +/-2，894.74纳克/克）。我们的结果表明，这些阔鼻动物分泌可测量浓度的DHEAS，其中A. caraya分泌水平相当于catarrhines。该试剂盒可用于粪便和血清中DHEAS的定量检测，对研究应激和灵长类进化具有重要意义。
The hormone dehydroepiandrosterone (DHEA) and its sulfate ester (DHEAS) are the most abundant circulating steroids in human and some nonhuman primates, and have been implicated in development, aging and in stress modulation. We aimed to validate a commercially available enzyme immunoassay kit to measure DHEAS in feces and serum of three platyrrhine primate species (Alouatta caraya, Aotus azarae infulatus, and Sapajus apella) in captivity. We collected serum samples from one male and one female from each species. To validate the kit for fecal samples, we conducted a physiological validation by administering DHEA orally to one adult female of each species. We also measured fecal DHEAS levels in four female Alouatta caraya individuals during the third semester of gestation and in two females following parturition. We obtained a total of 54 fecal samples and 6 serum samples from 10 individuals. We validated the assay analytically by testing parallelism and accuracy tests in both fecal and serum extracts for all species. We observed a peak in DHEAS 24 h following oral DHEA administration in all three species, with A. caraya presenting the strongest response and highest baseline concentrations. DHEAS levels were elevated in pregnant A. caraya (57,843.86 +/- 37,160.31 ng/g) and declined after parturition (1,539.07 +/- 2,894.74 ng/g). Our results demonstrated that these platyrrhines secrete measurable concentrations of DHEAS, with A. caraya secreting levels comparable to those of catarrhines. The EIA kit is valid for quantification of fecal and serum DHEAS, and it is useful for studies on stress and primate evolution.