Induction of calcification in MC3T3-E1 cells by inorganic polyphosphate
Induction of calcification in MC3T3-E1 cells by inorganic polyphosphate
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DOI：
10.1177/154405910408300806
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发表时间：
2004-08-01
影响因子：
7.6
通讯作者：
Kohgo, T
中科院分区：
文献类型：
--
作者：
Kawazoe, Y;Shiba, T;Kohgo, T
在正常成骨细胞中发现了相对大量的无机多磷酸盐[poly（P）]（400 μ M）。因此，使用MC 3 T3-E1细胞研究了平均链长为65个磷酸残基的聚（P）对细胞钙化的影响。骨桥蛋白和骨钙素的表达由聚（P）（0.1类似于1 mM）诱导，用聚（P）处理的细胞被茜素红强烈染色。此外，在聚（P）处理的细胞中诱导的碱性磷酸酶活性的水平比正磷酸盐处理或对照细胞中的高两倍，但不高于用β-甘油磷酸盐和抗坏血酸处理的细胞中的活性。然而，与此相反，聚磷酸酶活性激活聚（P）处理的水平高达六倍以上的控制。MC 3 T3-E1细胞可能利用聚（P）作为钙化的磷酸盐来源，而不是主要由ALP 4产生的磷酸盐来源。因此，多聚磷酸酶活性的多聚（P）依赖性诱导可能促进MC 3 T3-E1细胞的钙化。
Relatively large amounts of inorganic polyphosphate [poly(P)] (400 muM) have been found in normal osteoblasts. The effect of poly( P) with an average chain length of 65 phosphate residues on cell calcification was therefore investigated with the use of MC3T3-E1 cells. Expression of both osteopontin and osteocalcin was induced by poly(P) (0.1 similar to 1 mM), and cells treated with poly(P) were strongly stained by alizarin red. In addition, the level of alkaline phosphatase activity induced in poly(P)-treated cells was two-fold higher than that in either orthophosphate-treated or control cells but not higher than that in cells treated with beta-glycerophosphate and ascorbic acid. In contrast, however, polyphosphatase activities were activated by poly(P) treatment to levels up to sixfold greater than that in controls. MC3T3-E1 cells may utilize poly(P) as a phosphate source for calcification rather than phosphate sources that are mainly produced by ALPase. Poly(P)-dependent induction of polyphosphatase activities may therefore promote calcification in MC3T3-E1 cells.