HIGH-LEVELS OF HIV-1 IN PLASMA DURING ALL STAGES OF INFECTION DETERMINED BY COMPETITIVE PCR
HIGH-LEVELS OF HIV-1 IN PLASMA DURING ALL STAGES OF INFECTION DETERMINED BY COMPETITIVE PCR
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DOI：
10.1126/science.8096089
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发表时间：
1993-03-19
期刊：
影响因子：
56.9
通讯作者：
LIFSON, JD
中科院分区：
文献类型：
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作者：
PIATAK, M;SAAG, MS;LIFSON, JD
采用定量竞争性聚合酶链反应 (QC-PCR) 方法对 66 名疾病控制中心 I 期至 IVC1 感染患者血浆中病毒体相关人类免疫缺陷病毒 1 型 (HIV-1) RNA 进行定量。 HIV-1 RNA 的范围为每毫升血浆 100 到近 22,000,000 个拷贝（相当于每毫升 50 到 11,000,000 个病毒粒子），在所有受试者中都很容易定量，与疾病阶段和 CD4+ T 细胞计数显着相关，并且随着原发感染的解决或抗逆转录病毒治疗的开始而减少多达 235 倍。 QC-PCR 测定的血浆病毒水平与终点稀释培养测定的病毒滴度相关，但平均超过 60,000 倍。通过 QC-PCR 对血浆中的 HIV-1 进行定量可能有助于评估抗逆转录病毒药物的疗效，特别是在疾病早期，此时传统病毒标记物通常呈阴性。
Quantitative competitive polymerase chain reaction (QC-PCR) methods were used to quantify virion-associated human immunodeficiency virus type-1 (HIV-1) RNA in plasma from 66 patients with Centers for Disease Control stage I to IVC1 infection. HIV-1 RNA, ranging from 100 to nearly 22,000,000 copies per milliliter of plasma (corresponding to 50 to 11,000,000 virions per milliliter), was readily quantified in all subjects, was significantly associated with disease stage and CD4+ T cell counts, and decreased by as much as 235-fold with resolution of primary infection or institution of antiretroviral therapy. Plasma virus levels determined by QC-PCR correlated with, but exceeded by an average of 60,000-fold, virus titers measured by endpoint dilution culture. Quantitation of HIV-1 in plasma by QC-PCR may be useful in assessing the efficacy of antiretroviral agents, especially in early stage disease when conventional viral markers are often negative.