Overexpression of the maize WRKY114 gene in transgenic rice reduce plant height by regulating the biosynthesis of GA
Overexpression of the maize WRKY114 gene in transgenic rice reduce plant height by regulating the biosynthesis of GA
复制标题
转基因水稻中玉米WRKY114基因的过度表达通过调节GA的生物合成降低株高
DOI：
10.1080/15592324.2021.1967635
复制
发表时间：
2021-09-10
影响因子：
2.9
通讯作者：
Cai, Ronghao
中科院分区：
文献类型：
--
作者：
Fang, Xiu;Bo, Chen;Cai, Ronghao
WRKYs是一个重要的转录因子家族，广泛参与植物的发育、防御调节和胁迫反应。组成型表达ZmWRKY 114的转基因水稻株高较低，对赤霉酸（GA（3））的敏感性较低。进一步的研究表明，转基因水稻积累的生物活性GAs水平低于野生型植株。外源GA（3）的施用完全挽救了ZmWRKY 114转基因植株的半矮秆表型。转录组和qRT-PCR分析表明，编码GA生物合成阻遏物的OsGA 2 ox 4的表达显著增加。电泳迁移率变动分析和双荧光素酶报告基因分析表明ZmWRKY 114与OsGA 2 ox 4启动子中的W-box基序直接结合。综上所述，这些结果证实了ZmWRKY 114是一个GA响应基因，参与了水稻株高的调控。
WRKYs represent an important family of transcription factors that are widely involved in plant development, defense regulation and stress response. Transgenic rice that constitutively expressed ZmWRKY114 had shorter plant height and showed less sensitivity to gibberellic acid (GA(3)). Further investigation proved that transgenic rice accumulated lower levels of bioactive GAs than that in wild-type plants. Application of exogenous GA(3) fully rescued the semi-dwarf phenotype of ZmWRKY114 transgenic plants. Transcriptome and qRT-PCR analyses indicated that the expression of OsGA2ox4, encoding the repressor of GA biosynthesis, was markedly increased. Electrophoretic mobility shift assay and dual-luciferase reporter assay indicated that ZmWRKY114 directly binds to a W-box motif in the OsGA2ox4 promoter. Taken together, these results confirm that ZmWRKY114 is a GA-responsive gene and is participated in the regulation of plant height in rice.