RANKL and OPG expression: Jiggling force affects root resorption in rats.
RANKL and OPG expression: Jiggling force affects root resorption in rats.
复制标题
DOI：
10.2319/102515-718.1
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yayoi Matsuda;M. Motokawa;M. Kaku;H. Sumi;K. Tanne;K. Tanimoto
中科院分区：
文献类型：
--
作者：
Yayoi Matsuda;M. Motokawa;M. Kaku;H. Sumi;K. Tanne;K. Tanimoto
目的用免疫组织化学方法观察实验动物模型牙根屈曲力时牙根吸收的纵向变化。材料与方法选用12周龄雄性Wistar大鼠56只。28只大鼠(实验组)用实验矫治器沿颊舌向交替移动上颌第一磨牙，产生10g的摆动力，另28只大鼠(对照组)仅沿舌向移动上颌第一磨牙10g，分别于1、3、7、10、14、17、21d切取上颌骨进行免疫组织化学染色。采用组织形态计量学方法对牙根吸收面积进行定量，并对牙根表面破牙细胞数量进行计数。免疫组织化学染色检测RANKL和OPG的表达。结果实验组牙根吸收面积和破牙细胞数均明显大于对照组。实验组牙骨质吸收陷窝和牙周韧带内可见破骨细胞，而对照组破骨细胞仅沿牙槽骨方向分布。实验组牙槽骨和对照组牙根表面均可检测到OPG。结论摇摆力是严重牙根吸收的关键因素，影响RANKL和OPG的表达，分别促进和抑制破牙诱导。
OBJECTIVE To immunohistochemically investigate the longitudinal changes in root resorption by jiggling force in experimental animal models. MATERIALS AND METHODS Fifty-six 12-week-old male Wistar rats were used. The maxillary first molars were alternately moved in the buccal and lingual direction in 28 rats (experimental group) using an experimental appliance to produce jiggling forces of 10 g. In another 28 rats (control group), the maxillary first molars were moved in only the lingual direction with a force of 10 g. After 1, 3, 7, 10, 14, 17, and 21 days, the maxillae were resected and subjected to immunohistochemical analysis. The resorption area was quantified histomorphometrically and the number of odontoclasts on the root surface was counted. Expression of RANKL and OPG was also examined by immunohistochemical staining. RESULTS The root resorption area and the number of odontoclasts were significantly greater in the experimental group than in controls. Odontoclasts were detected in the resorption lacunae and PDL in the experimental group, whereas osteoclasts were located only along the alveolar bone in controls. OPG was detected on the alveolar bone in the experimental group and on the root surfaces of the controls. CONCLUSIONS Jiggling force is a critical factor in severe root resorption, affecting RANKL and OPG expression, which accelerates and inhibits odontoclastic induction, respectively.