Multiplexed immunofluorescence microscopy for the interrogation of cellular protein complexes
Multiplexed immunofluorescence microscopy for the interrogation of cellular protein complexes
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用于研究细胞蛋白复合物的多重免疫荧光显微镜
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
T. Veenstra
中科院分区：
文献类型：
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作者：
Ming Zhou;T. Veenstra
评价：Schubert W，Bonnekoh B，Pommer AJ等。通过自动多维荧光显微镜分析蛋白质组拓扑结构和功能。Nature Biotechnology 24（10），1270 - 1278（2006）1.知道一种特定的蛋白质存在于细胞内，对它的功能几乎没有帮助。在Schubert及其同事的一项研究中，研究人员提出了一种利用荧光显微镜和自动抗体引入和检测的多维方法，该方法可能能够定位单个细胞内的数百种蛋白质。该方法，称为多表位配体制图，在外周血单核细胞中的细胞表面受体的分析中得到验证，然后用于映射一系列疾病模型中的蛋白质复合物，包括银屑病和慢性压迫性损伤。在每个实验中，每个蛋白质的区域以二进制格式呈现，并解释数据以识别控制蛋白质网络拓扑结构的特定蛋白质。希望是通过确定蛋白质和那些最负责这些相互作用的蛋白质之间的伙伴关系，可以建立新的诊断特征和治疗靶点。
Evaluation of: Schubert W, Bonnekoh B, Pommer AJ et al. Analyzing proteome topology and function by automated multidimensional fluorescence microscopy. Nature Biotechnology 24(10), 1270–1278 (2006) 1. Knowing that a specific protein is present within a cell provides little insight into its function. In a study by Schubert and colleagues, the investigators present a multidimensional method that utilizes fluorescence microscopy and automated antibody introduction and detection, which is potentially capable of localizing hundreds of proteins within individual cells. The method, referred to as multiepitope-ligand cartography, is validated in the analysis of cell-surface receptors in peripheral mononuclear blood cells, and then used to map protein complexes in a series of disease models, including psoriasis and chronic constriction injury. Within each experiment, the locales of each protein are presented in a binary format and the data are interpreted to recognize specific proteins that control the topology of the protein network. The hope is that by identifying partnerships between proteins and those proteins that are most responsible for these interactions, novel diagnostic features and therapeutic targets can be established.