Electron microscopic localization of Alzheimer neurofibrillary tangle components recognized by an antiserum to paired helical filaments.
Electron microscopic localization of Alzheimer neurofibrillary tangle components recognized by an antiserum to paired helical filaments.
复制标题
抗血清识别的阿尔茨海默氏症神经原纤维缠结成分的电子显微镜定位与成对的螺旋丝。
DOI：
10.1097/00005072-198603000-00006
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发表时间：
1986
影响因子：
3.2
通讯作者：
Gambetti,P
中科院分区：
文献类型：
--
作者：
Perry,G;Selkoe,DJ;Block,BR;Stewart,D;Autilio-Gambetti,L;Gambetti,P
配对螺旋细丝（PHF）是阿尔茨海默神经原纤维缠结的主要成分；然而，其他元素，如10和15 nm的直细丝和粒状非晶成分，被观察到。在本研究中，通过电镜免疫细胞化学检查了抗血清识别的抗原决定因子对不识别正常神经组织成分的PHF组分的定位。采用过氧化物酶-抗过氧化物酶程序对完整组织中的神经原纤维缠结，我们发现抗血清与PHF和缠结内的粒状无定形物质发生反应。在用离子洗涤剂处理和免疫金标记分离PHF后，抗原决定因子常常间隔出现，而不是沿PHF均匀分布。该抗血清识别的抗原决定因子的分布与先前观察到的神经丝和微管相关蛋白抗体识别的PHF决定因子的分布相似。我们得出结论，PHF是异质结构，包含PHF特异性以及细胞骨架衍生的抗原决定因子。
Paired helical filaments (PHF) are the main component of the Alzheimer neurofibrillary tangle; however, other elements, such as 10 and 15 nm straight filaments and granular amorphous components, are observed. In the present study, the localization of the antigenic determinants recognized by an antiserum to PHF fractions that does not recognize normal nervous tissue components is examined by electron microscopic immunocytochemistry. Employing the peroxidase-antiperoxidase procedure on neurofibrillary tangles in intact tissue, we found that the antiserum reacts with PHF and with granular amorphous material within the tangle. Following isolation of PHF by treatment with ionic detergent and labeling with immunogold, the antigenic determinants often occur at intervals rather than being uniformly distributed along the PHF. The distribution of the antigenic determinants recognized by thisantiserum is similar to that previously observed for PHF determinants recognized by antibodies to neurofilament and microtubule-associated proteins. We conclude that PHF are heterogenous structures which contain PHF-specific as well as cytoskeleton-derived antigenic determinants.