Supersensitive single-particle plasmonic scattering-based cancer antigen 125 nanoimmunosensor by enhanced dark-field microscopy with dual-detection mode
Supersensitive single-particle plasmonic scattering-based cancer antigen 125 nanoimmunosensor by enhanced dark-field microscopy with dual-detection mode
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DOI：
10.1016/j.snb.2017.01.191
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发表时间：
2017-06
影响因子：
8.4
通讯作者：
Soyeong Ju;Suresh Kumar Chakkarapani;Seungah Lee;S. Kang
中科院分区：
文献类型：
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作者：
Soyeong Ju;Suresh Kumar Chakkarapani;Seungah Lee;S. Kang
癌症抗原 125 (CA125) 是多种癌症的阶段标志物，其超灵敏纳米免疫传感器是基于双检测模式的波长依赖性增强暗场显微镜 (EDFM-DM) 对单粒子等离子体散射的表征而开发的。考虑到金银纳米粒子 (AuNPs/AgNPs) 的光学特性，选择 40 nm AgNP 作为最佳无荧光探针。在双检测模式下，使用在金纳米点上免疫反应的 40 nm AgNP 标记的 CA125 的波长依赖性等离子体散射强度来分析 CA125。基于暗场散射图像，分别使用彩色数码相机和电子倍增电荷耦合器件相机进行定性和定量分析。在最佳条件下，所开发的免疫传感器的检测下限为4μU/mL（S/N=3），动态检测范围为4μU/mL-80U/mL（R=0.9935），比以前的方法检测限低100-375,000倍，动态范围宽100-100,000倍。此外，在人血清样品中添加标准 CA125 后，回收率大于 98%。该方法可能是一个优秀的纳米免疫分析平台，可在宽动态检测范围内进行超灵敏检测，支持单分子水平上各种疾病相关蛋白分子的早期检测。
A supersensitive nanoimmunosensor of cancer antigen 125 (CA125), which is a stage marker of several types of cancers, was developed based on characterization of single-particle plasmonic scattering by wavelength-dependent enhanced dark-field microscopy with dual-detection mode (EDFM-DM). Considering the optical properties of gold and silver nanoparticles (AuNPs/AgNPs), 40-nm AgNP was selected as an optimal fluorescence-free probe. CA125 was analyzed using the wavelength-dependent plasmonic scattering intensity of 40-nm AgNP-labeled CA125 immunoreacted on gold nanodots under the dual-detection mode. A color digital camera and an electron multiplying charge-coupled device camera were used for qualitative and quantitative analysis, respectively, based on the dark-field scattering images. Under optimal conditions, the developed immunosensor exhibited a lower detection limit of 4 μU/mL (S/N= 3) with a wide dynamic detection range of 4 μU/mL-80 U/mL (R= 0.9935), which was a 100–375,000-fold lower detection limit with a 100–100,000-fold wider dynamic range than previous methods. In addition, recovery was greater than 98% with the spiking of standard CA125 in human serum samples. This method may be an excellent nanoimmunoassay platform for supersensitive detection at a wide dynamic detection range, supporting the earliest-stage detection of various disease-related protein molecules at the single-molecule level.