BcMF20, a putative pollen-specific transcription factor from Brassica campestris ssp chinensis

BcMF20, a putative pollen-specific transcription factor from Brassica campestris ssp chinensis
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DOI:
10.1007/s11033-011-0682-9
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发表时间:
2011-11-01
影响因子:
2.8
通讯作者:
Cao, Jiashu
Cao, Jiashu
中科院分区:
生物学4区
文献类型:
--
作者:
Han, Yingyan;Zhang, Aihong;Cao, Jiashu

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结果表明,白菜雄性不育系与保持系相比,拟南芥转录因子(At1g26610)表达下调。在我们之前的研究中。从大白菜中分离到与At1g26610基因同源的BcMF20基因。它编码一种推定的C2H2锌指蛋白。qRT-PCR和原位杂交分析结果表明,BcMF20特异表达于单核花粉期至成熟花粉期的花粉粒和绒毡层中。
The Arabidopsis transcription factor (At1g26610) was shown to be down-regulated in male sterile lines compared to their maintainer line in Chinese cabbage (Brassica campestris ssp. chinensis) in our previous study. The BcMF20 gene, homologue of the At1g26610 gene was isolated from Chinese cabbage. It encodes a putative C2H2 zinc finger protein. The spatial and temporal expression patterns examined by qRT-PCR and in situ hybridization, indicated BcMF20 is specifically expressed in the developing pollen grains and the tapetum from the uninucleate pollen stage to mature pollen stage.