Distribution of both lengths and 5' terminal nucleotides of mammalian pre-tRNA 3' trailers reflects properties of 3' processing endoribonuclease
Distribution of both lengths and 5' terminal nucleotides of mammalian pre-tRNA 3' trailers reflects properties of 3' processing endoribonuclease
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DOI：
10.1093/nar/25.6.1148
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发表时间：
1997-03-15
影响因子：
14.9
通讯作者：
Nashimoto, M
中科院分区：
文献类型：
--
作者：
Nashimoto, M
哺乳动物tRNA 3‘加工核糖核酸内切酶（3’ tRNase）从tRNA前体的鉴别器后去除3'额外的核苷酸。我检查的长度3的预告片和裂解位点的核苷酸两侧影响3的处理效率,进行体外3的处理反应pre-tRNA (Arg)年代各种3预告片或各种鉴别器核苷酸通过3’tRNase纯化从老鼠FM3A细胞或猪的肝脏,总的来说,前置tRNA的效率(Arg) 3 '处理哺乳动物3 tRNase减少为3的预告片变得更长,除了3 '的情况下拖车组成的CC,从哺乳动物核tRNA基因组序列中推断出的3‘尾链长度分布反映了3’ tRNase的这一特性，前tRNA(Arg)s的切割效率取决于3‘尾链的5’端核苷酸的G顺序，类似于> U > c, 5'端核苷酸的影响与鉴别器核苷酸无关。哺乳动物trna - 3‘预告片的5’端核苷酸分布反映了这种差异的3'加工效率。
Mammalian tRNA 3' processing endoribonuclease (3' tRNase) removes 3' extra nucleotides after the discriminator from tRNA precursors. Here I examined how the length of a 3' trailer and the nucleotides on each side of the cleavage site affected 3' processing efficiency, I performed in vitro 3' processing reactions of pre-tRNA(Arg)s with various 3' trailers or various discriminator nucleotides using 3' tRNase purified from mouse FM3A cells or pig liver, On the whole, the efficiency of pre- tRNA(Arg) 3' processing by mammalian 3' tRNase decreased as the 3' trailer became longer, except in the case of a 3' trailer composed of CC, CCA or CCA plus 1 or 2 nucleotides, which was not able to be removed at all, The distribution of 3' trailer lengths deduced from mammalian nuclear tRNA genomic sequences reflects this property of 3' tRNase, The cleavage efficiency of pre-tRNA(Arg)s varied depending on the 5' end nucleotide of a 3' trailer in the order G similar to > U > C. This effect of the 5' end nucleotide was independent of the discriminator nucleotides. The distribution of the 5' end nucleotides of mammalian pre-tRNA 3' trailers reflects this differential 3' processing efficiency.