Tissue-Adhesive Decellularized Extracellular Matrix Patches Reinforced by a Supramolecular Gelator to Repair Abdominal Wall Defects
Tissue-Adhesive Decellularized Extracellular Matrix Patches Reinforced by a Supramolecular Gelator to Repair Abdominal Wall Defects
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超分子凝胶剂增强的组织粘附脱细胞细胞外基质补片修复腹壁缺损
DOI：
10.1021/acs.biomac.2c01210
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发表时间：
2023
影响因子：
6.2
通讯作者：
Taguchi Tetsushi
中科院分区：
文献类型：
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作者：
Nishiguchi Akihiro;Ito Shima;Nagasaka Kazuhiro;Taguchi Tetsushi
由人工合成材料和生物材料组成的外科网片已应用于腹壁缺损的修复。尽管做了许多努力，但由于缺乏生物降解性、机械强度和组织粘附性，目前还没有完全满足临床要求的可靠网状物。在这里，我们报告了可生物降解、脱细胞的细胞外基质(DECM)为基础的生物补片治疗腹壁缺陷。通过加入不溶于水的超分子凝胶剂，通过分子间氢键形成物理交联网络，dECM贴片得到增强，以提高机械强度。与原dECM相比，增强的dECM贴片具有更高的组织黏附强度和水下稳定性，这是因为增强的dECM贴片的界面黏附强度增强。采用大鼠腹壁缺损模型进行的体内实验表明，与不可生物降解的合成网状物相比，增强的dECM贴片在材料降解过程中诱导胶原沉积和血管形成，并抑制CD68阳性巨噬细胞的聚集。组织粘附性和生物可降解性超分子凝胶器提高了ECM的机械强度，在修复腹壁缺损方面具有巨大的潜力。
Implantation of surgical meshes composed of synthetic and biological materials has been applied for abdominal wall defect repair. Despite many efforts, there are no reliable meshes that fully satisfy clinical requirements because of their lack of biodegradability, mechanical strength, and tissue-adhesive properties. Here, we report biodegradable, decellularized extracellular matrix (dECM)-based biological patches to treat abdominal wall defects. By incorporating a water-insoluble supramolecular gelator that forms physical cross-linking networks through intermolecular hydrogen bonding, dECM patches were reinforced to improve mechanical strength. Reinforced dECM patches possessed higher tissue adhesion strength and underwater stability compared with the original dECM because of enhanced interfacial adhesion strength. In vivo experiments using an abdominal wall defect rat model showed that reinforced dECM patches induced collagen deposition and the formation of blood vessels during material degradation, and the accumulation of CD68-positive macrophages was suppressed compared to nonbiodegradable synthetic meshes. Tissue-adhesive and biodegradable dECM patches with improved mechanical strength by a supramolecular gelator have enormous potential for use in the repair of abdominal wall defects.